Evidence map›Paper›PMID 41418777›Full record

ArticleStructure (London, England : 1993)2026

Structural insights into zinc piracy by Neisseria gonorrhoeae to overcome nutritional immunity.

Pooneh Tavakoley Gheinani, Aloke Bera, Julie Stoudenmire-Saylor, Yi Lien, Simone A Harrison, Alison Criss, Walter J Chazin, Nicholas Noinaj, Cynthia Nau Cornelissen

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Structure (London, England : 1993), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Pooneh Tavakoley GheinaniCenter for Translational Immunology, Institute for Biomedical Sciences, Georgia State University, Atlanta GA 30303, USA.
Aloke BeraDepartment of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA.
Julie Stoudenmire-SaylorCenter for Translational Immunology, Institute for Biomedical Sciences, Georgia State University, Atlanta GA 30303, USA.
Yi LienDepartment of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA.
Simone A HarrisonDepartments of Biochemistry and Chemistry, and Center for Structural Biology, Vanderbilt University, Nashville TN 37240, USA.
Alison CrissDepartment of Microbiology, Immunology, and Cancer Biology, University of Virginia, Charlottesville VA 22908, USA.
Walter J ChazinDepartments of Biochemistry and Chemistry, and Center for Structural Biology, Vanderbilt University, Nashville TN 37240, USA.
Nicholas NoinajDepartment of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA; Purdue Institute for Inflammation, Immunology and Infectious Disease, Purdue University, West Lafayette, IN 47907, USA. Electronic address: nnoinaj@purdue.edu.
Cynthia Nau CornelissenCenter for Translational Immunology, Institute for Biomedical Sciences, Georgia State University, Atlanta GA 30303, USA. Electronic address: ccornelissen@gsu.edu.

Funding

Quality Assurance and Quality Control Project Management: Improving Submissions and Study Conduct in the Human Subjects Research Prior Approval ProcessUL1TR002529 · NCATS · INDIANA UNIVERSITY INDIANAPOLIS · PI MOE, SHARON M, WIEHE, SARAH ELIZABETH · 2018 to 2022
$27.2M
Using gonococcal TonB-dependent transporters as vaccine antigensU19AI144182 · NIAID · GEORGIA STATE UNIVERSITY · PI CORNELISSEN, CYNTHIA N · 2019 to 2023
$9.3M
Neisseria gonorrhoeae metal transporters that subvert nutritional immunityR01AI127793 · NIAID · VIRGINIA COMMONWEALTH UNIVERSITY · PI CYNTHIA N CORNELISSEN, Alison K Criss · 2017 to 2026
$6.8M
NCATS NIH HHS UL1 TR002529NIAID NIH HHS R01 AI127793NIAID NIH HHS U19 AI144182
6 · The paper itself

Abstract

With an estimated 106 million global cases annually, Neisseria gonorrhoeae (Ngo) poses a significant public health problem. Ngo demonstrates a remarkable capacity to overcome nutritional immunity through the deployment of specialized transporters that pirate metals such as zinc from human proteins such as calprotectin (hCP). We report the cryo-EM structure of Ngo TdfH in complex with a heterotetramer of hCP. An extensive binding interface is mediated almost entirely by the S100A9 subunits of hCP. Mutagenesis studies of residues in the large binding interface reveal minimal effects from single-site mutations, whereas larger truncations disrupt binding and function. These results support a mechanistic model based on the large interaction interface overcoming a steric clash between α-helix III of S100A9 and loops 5 and 9 of TdfH, which leads to the distortion of the proximal His6 site, followed by zinc release, and import through the barrel domain.

Indexed as

Bacterial ProteinsLeukocyte L1 Antigen ComplexNeisseria gonorrhoeaeZincBinding SitesCryoelectron MicroscopyHumansModels, MolecularProtein BindingBacterial ProteinsLeukocyte L1 Antigen ComplexZinccalprotectinhost pathogen interactionsmembrane proteinNeisserianutritional immunity

Identifiers

PMID41418777
PMCPMC12858431

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.