ArticleApplied microbiology and biotechnology2025
Toluene-2-monooxygenase expression and trichloroethene oxidizing activity of Burkholderia vietnamiensis G4 grown on benzyl alcohol and benzyl esters.
Article in Applied microbiology and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Benzyl alcohol (BA) and two BA esters were examined for their ability to support growth of Burkholderia vietnamiensis G4 and expression of toluene-2-monooxygenase (T2MO) activity. Resting cell assays and an activity-based labeling (ABL) approach were used to quantify T2MO activity and levels of T2MO hydroxylase subunit in cells grown either on substrates already known to support T2MO expression (toluene, phenol); novel aromatic substrates (BA, benzyl acetate (BAc); benzyl butyrate (BBu)); and non-aromatics (lactate, acetate, and butyrate). Specific rates of TCE oxidation and levels of activity-based fluorescent labeling of T2MO hydroxylase α-subunits in cells grown on BA or BA esters were comparable to or greater than those of cells grown on toluene or phenol. In contrast, levels of activity and protein labeling of cells grown on acetate or butyrate were like those of lactate-grown cells. Cells grown on BA also degraded 1,2-cis-dichloroethene (cis-DCE), 1,1-dichloroethene (1,1-DCE), and vinyl chloride (VC). While toluene-grown cells generally exhibited higher specific rates of chloroethene transformation, BA-grown cells had consistently higher transformation capacities for these compounds. Stable accumulation of BA in batch cultures grown on BBu in the presence of propyne and the inhibitory effects of BA on TCE transformation both suggest BA is a T2MO substrate. Substrate-specific O
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.