ArticleEuropean journal of applied physiology2026
Effect of ischemic postconditioning on inflammatory and oxidative markers in methadone-treated rats during myocardial ischemia-reperfusion injury : Cardioprotective effects of IPoC in methadone exposure.
Article in European journal of applied physiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
purposeIschemia-reperfusion injury (IRI) poses a major challenge in cardiovascular therapy due to inflammation and oxidative stress. Ischemic postconditioning (IPoC) offers cardioprotection against IRI, but its efficacy may be compromised by chronic methadone use. This study examined the impact of IPoC on inflammatory and oxidative biomarkers in methadone-treated rats subjected to myocardial IRI.
methodsTwenty-four male Wistar rats were divided into four groups: IRI, methadone-treated IRI (IRI-M), IPoC-treated IRI (IRI-IPoC), and methadone-treated IPoC-IRI (IRI-M-IPoC). Inflammatory proteins (IL-1β, IL-6, TNF-α), oxidative markers (SOD, GPx, TAS), and MDA levels were measured using commercial kits. Gene expression of inflammatory and antioxidant (NRF2, SOD, and GPx) was measured using Real-time PCR assay.
resultsIPoC significantly reduced the expression of inflammatory genes (IL-1β, IL-6, TNF-α, NF-κB) and enhanced antioxidant responses (NRF2, SOD, GPx) in rats subjected to myocardial IRI. Methadone-treated rats (IRI-M) exhibited elevated inflammatory markers and suppressed antioxidant gene expression. The combination group (IRI-M-IPoC) showed partial restoration of antioxidant activity and moderate reduction in inflammation, but these effects were significantly weaker than those observed in the IPoC-only group.
conclusionIPoC effectively reduces myocardial IRI by modulating inflammation and oxidative stress. However, chronic methadone use impairs these protective effects, likely through redox imbalance and enhanced inflammatory signaling. These findings confirm the need for optimized therapeutic strategies, potentially including antioxidant support, for patients on opioid maintenance therapy. Further research is warranted to clarify the molecular interplay between opioids and conditioning interventions.
Indexed as
Identifiers
41417066What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.