Evidence map›Paper›PMID 41416528›Full record

ArticleSmall (Weinheim an der Bergstrasse, Germany)2026

A Simple, Ultrastable, and Cost-Effective Oxygen-Scavenging System for Long-Term DNA-PAINT Imaging.

Rebecca T Perelman, George M Church, Johannes Stein

Abstract read
In one paragraph

Article in Small (Weinheim an der Bergstrasse, Germany), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

3 authors.

Rebecca T PerelmanWyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, USA.ORCID https://orcid.org/0009-0003-7253-1118
George M ChurchWyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, USA.ORCID https://orcid.org/0000-0001-6232-9969
Johannes SteinWyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, USA.ORCID https://orcid.org/0000-0002-1335-1120

Funding

Center for Genome ImagingRM1HG011016 · NHGRI · HARVARD MEDICAL SCHOOL · PI WU, CHAO-TING · 2021 to 2025
$12.2M
European Molecular Biology Organization ALTF 816-2021National Science FoundationNHGRI NIH HHS RM1 HG011016NIH HHS 5RM1HG011016-03U.S. Department of Energy DE-FG02-02ER63445
6 · The paper itself

Abstract

DNA-PAINT (Points Accumulation in Nanoscale Topography) is a super-resolution microscopy technique capable of nanoscale imaging through the transient binding of fluorescently labeled imager strands to complementary DNA docking strands. Imager strands can be continuously replenished from an effectively infinite pool, making DNA-PAINT inherently resistant to photobleaching. However, extended DNA-PAINT imaging is limited by the formation of reactive oxygen species (ROS), which damage docking strands and reduce localization sampling over time. Although the state-of-the-art oxygen-scavenging system (OSS) can mitigate this damage, its enzymatic components degrade over time, reducing its performance, robustness, and utility. Here, we introduce a simple, enzyme-free oxygen scavenging buffer based on sodium sulfite (Na

Indexed as

DNAOxygenChromansCost-Benefit AnalysisReactive Oxygen SpeciesSulfites6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acidChromansDNAOxygenReactive Oxygen SpeciesSulfitesDNA‐PAINToxygen‐scavengersingle‐moleculesuper‐resolution microscopy

Identifiers

PMID41416528
PMCPMC12895217

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.