Field phenotyping for soybean density tolerance using time-series prediction and dynamic modeling.

With the increasing global demand for food, breeding soybean varieties resistant to dense planting is crucial for achieving high and stable yields. Traditional phenotyping methods are limited by insufficient temporal resolution and challenges in dynamic modeling continuity, making it difficult to elucidate the intrinsic relationship between canopy development rate and yield stability. Moreover, existing machine learning models often neglect temporal dependencies in time series predictions, leading to insufficient biological interpretability. This study proposes an innovative approach integrating spatiotemporal deep learning and dynamic modeling to quantify the dynamic changes in canopy parameters using UAV high-throughput phenotyping technology, revealing the key regulatory mechanisms of traits associated with resistance to dense planting. Based on a two-year field experiment (2022-2023) in northeast China (Qiqihaer, black soil region), this study set high (50w plants/ha) and low density (30w plants/ha) treatments across 208 soybean varieties, combined with multispectral UAV imagery (15-18 times per season) and ground-truth data, to develop a time series prediction model for leaf area index (LAI). Comparing the performance of spatiotemporal residual networks (ST-ResNet), long short-term memory networks (LSTM), and traditional random forests (RF), the ST-ResNet model demonstrated significantly superior prediction accuracy (R