Evidence map›Paper›PMID 41416160›Full record

ArticleSichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition2025

[From the [Formula: see text]Method to the [Formula: see text]Method: A More Rigorous Approach to Real-time Quantitative Polymerase Chain Reaction Data Analysis].

Lixiang Feng, Rongqian Zhao, Kui Zhang, Wenxing Yang

Abstract readEnglish Abstract
PubMed Full text at PMC Publisher
In one paragraph

Article in Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Lixiang Feng（ 610041） Department of Physiology, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu 610041, China.ORCID 0009-0005-2191-173X
Rongqian Zhao（ 610041） Department of Physiology, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu 610041, China.
Kui Zhang（ 610041） Department of Physiology, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu 610041, China.
Wenxing Yang（ 610041） Department of Physiology, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu 610041, China.ORCID 0000-0003-0965-787X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Objective: To optimize the real-time quantitative polymerase chain reaction (RT-qPCR) data analysis process through mathematical principles by replacing the biased [Formula: see text] method with a more rigorous [Formula: see text] method, thereby improving the accuracy of gene expression quantification analysis. Methods: Essentially, the Results: In the original dataset from LIVAK and SCHMITTGEN, the two methods produced similar results. However, in the cadmium exposure experiment, findings from the [Formula: see text] method indicated that 8-hour cadmium exposure caused an increase of Conclusion: The [Formula: see text] method provides a mathematically more rigorous approach that more accurately reflects gene expression changes, particularly in experiments with high

Indexed as

Real-Time Polymerase Chain ReactionAlgorithmsAnimalsData AnalysisGene Expression Profiling$ 2^{-C_{\mathrm{T}}} $$ 2^{-\Delta\Delta C_{\mathrm{T}}} $CadmiumCaenorhabditis elegansData analysisGene expression quantificationirg-6RT-qPCR

Identifiers

PMID41416160
PMCPMC12709095

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.