Evidence map›Paper›PMID 41415406›Full record

ArticlebioRxiv : the preprint server for biology2025

Degradation factor 1, Def1, regulates mRNA translation and decay through Ccr4-Not-dependent ubiquitylation of the ribosome.

O T Akinniyi, A Sebastian, S Kulkarni, I Albert, J C Reese

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors.

O T AkinniyiDepartment of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA, 16802 USA.
A SebastianHuck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA, 16802 USA.
S KulkarniDepartment of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA, 16802 USA.
I AlbertDepartment of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA, 16802 USA.
J C ReeseDepartment of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA, 16802 USA.

Funding

Activities of yeast Ccr4-Not transcription factor complex - SupplementR35GM136353 · NIGMS · PENNSYLVANIA STATE UNIVERSITY, THE · PI REESE, JOSEPH C · 2020 to 2024
$2.0M
Eukaryotic Gene Regulation (EGR) Predoctoral Training ProgramT32GM125592 · NIGMS · PENNSYLVANIA STATE UNIVERSITY, THE · PI REESE, JOSEPH C · 2018 to 2022
$920k
NIGMS NIH HHS R35 GM136353NIGMS NIH HHS T32 GM125592
6 · The paper itself

Abstract

Yeast Def1 is well known for its role in regulating RNA polymerase II elongation and degrading the large subunit of polymerase during transcriptional stress. It is an abundant cytoplasmic protein that undergoes stress-induced processing and is then transported to the nucleus. Previous research from our lab has shown that Def1 interacts with various proteins involved in mRNA decay and translation control, and that it regulates mRNA half-lives, suggesting an important role in the cytoplasm. In this study, we report that Def1 binds polyribosomes and that its null mutant strain exhibits phenotypes indicating a role in translation. Ribo-seq analysis revealed that deleting

Indexed as

Ccr4-Notco-translational decayDef1posttranscriptional controltranslationubiquitylation

Identifiers

PMID41415406
PMCPMC12710963

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.