Evidence map›Paper›PMID 41415364›Full record

ArticlebioRxiv : the preprint server for biology2025

Advancing Knock-In Approaches for Robust Genome Editing in Zebrafish.

Anjelica Rodriguez-Parks, Ella Grace Beezley, Steffani Manna, Isabella Silaban, Sarah I Almutawa, Siyang Cao, Hossam Ahmed, Megan Guyer, Sean Baker, Mark P Richards and 1 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors.

Anjelica Rodriguez-ParksDepartment of Cell and Regenerative Biology, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, 53705, USA.
Ella Grace BeezleyDepartment of Cell and Regenerative Biology, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, 53705, USA.
Steffani MannaDepartment of Cell and Regenerative Biology, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, 53705, USA.
Isabella SilabanDepartment of Cell and Regenerative Biology, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, 53705, USA.
Sarah I AlmutawaDepartment of Cell and Regenerative Biology, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, 53705, USA.
Siyang CaoDepartment of Cell and Regenerative Biology, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, 53705, USA.
Hossam AhmedDepartment of Cell and Regenerative Biology, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, 53705, USA.
Megan GuyerDepartment of Cell and Regenerative Biology, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, 53705, USA.
Sean BakerDepartment of Animal and Dairy Sciences, University of Wisconsin-Madison, Madison, WI 53706, USA.
Mark P RichardsDepartment of Animal and Dairy Sciences, University of Wisconsin-Madison, Madison, WI 53706, USA.
Junsu KangDepartment of Cell and Regenerative Biology, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, 53705, USA.

Funding

UW COMPREHENSIVE CANCER CENTER SUPPORTP30CA014520 · NCI · UNIVERSITY OF WISCONSIN-MADISON · PI Lu Mao · 1985 to 2026
$142.6M
Molecular and Genetic Analysis of Fin Regeneration in ZebrafishR35GM137878 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI Junsu Kang · 2020 to 2026
$3.1M
Dissecting injury-responsive gene expression during zebrafish heart regenerationR01HL151522 · NHLBI · UNIVERSITY OF WISCONSIN-MADISON · PI Junsu Kang · 2021 to 2026
$2.6M
Developing robust genome editing tools for generating floxed alleles and editing amino acid change in zebrafishR21OD037634 · OD · UNIVERSITY OF WISCONSIN-MADISON · PI Junsu Kang · 2025 to 2026
$428k
NCI NIH HHS P30 CA014520NHLBI NIH HHS R01 HL151522NIGMS NIH HHS R35 GM137878NIH HHS R21 OD037634
6 · The paper itself

Abstract

Precise genome editing remains a major challenge in functional genomics, particularly for generating knock-in (KI) alleles in model organisms. Here, we introduce the mini-golden system, a versatile Golden Gate-based subcloning platform that enables rapid assembly of donor constructs containing homology arms and a gene of interest. This system offers a library of middle entry vectors including diverse genes, enhancing the preparation of donor minicircles for KI applications. Using the mini-golden system, we efficiently generated a

Identifiers

PMID41415364
PMCPMC12710763

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.