Evidence map›Paper›PMID 41414961›Full record

ArticleJournal of insect science (Online)2025

Multiplex PCR-based identification of two sympatric stem borer species, Sesamia cretica and Sesamia nonagrioides (Lepidoptera: Noctuidae).

Fatemeh Karimi, Asadollah Hosseini Chegeni, Zahra Mirzaeipour

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of insect science (Online), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Fatemeh KarimiDepartment of Plant Protection, Faculty of Agriculture, Lorestan University, Khorramabad, Iran.ORCID 0009-0006-9637-4913
Asadollah Hosseini ChegeniDepartment of Plant Protection, Faculty of Agriculture, Lorestan University, Khorramabad, Iran.ORCID 0000-0002-7494-8641
Zahra MirzaeipourDepartment of Plant Protection, Faculty of Agriculture, Lorestan University, Khorramabad, Iran.ORCID 0000-0002-1279-2696

Funding

Lorestan University
6 · The paper itself

Abstract

Stem borers of the genus Sesamia are major pests of cereals in tropical regions. In Iran, Sesamia cretica and Sesamia nonagrioides significantly affect maize and sugarcane, but distinguishing them at the larval stage is difficult due to morphological similarity. In this study, we developed and optimized a rapid, cost-effective multiplex PCR method for simultaneous identification of both species. Primers designed from GenBank sequences yielded specific bands of 367 bp for S. cretica and 438 bp for S. nonagrioides. PCR and sequencing confirmed the specificity of the designed primers, while morphological comparison of larvae and reared adults validated the molecular results. This molecular tool supports integrated pest management by improving identification accuracy, which could help reduce unnecessary pesticide applications.

Indexed as

MothsMultiplex Polymerase Chain ReactionAnimalsLarvaSpecies SpecificityCOI geneIranMolecular approachSesamia sppSpecies diagnosis

Identifiers

PMID41414961
PMCPMC12715308

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.