ArticleJournal of the American Chemical Society2026
What Determines the Directionality of Kinesins? Studying the Anomalous Directionality of Nonclaret Disjunctional Motor Mutants.
Article in Journal of the American Chemical Society, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
NCD (nonclaret disjunctional) motors are a class of kinesin family proteins that are known to walk toward the minus end along the microtubule. Understanding the origin of such unique directionality of NCD motors and related kinesins has been a problem of fundamental importance. Experimental studies have indicated that specific NCD motor mutants switch their directionality. A type of NCD mutant (ran12) walks in the opposite direction (plus end) compared to the wild type (WT), whereas another mutant (N340K) of NCD shows diffusive bidirectionality. This work establishes that the directionality of these NCD motors is regulated by the barrier of the rate-determining ADP release step. We explore the molecular origin of the anomalous directionality of the mutated NCD motors compared with the wild type and determine the change in the barriers of ADP release for forward and backward motions. Our investigation is performed by combining different simulation techniques such as coarse-grained (CG) modeling, all-atom steered molecular dynamics (SMD) simulation, free energy estimation, as well as structural and stability analysis, and the maximum entropy (MaxEnt) method. We conclude that the differences in the directionality of different types of NCD motors are controlled by the free energy barrier for the ADP release step. Finally, we show that the statistical energy deduced from homologous sequences by the maximum entropy (MaxEnt) principle is significantly correlated with the stability and experimentally measured velocities of the wild type and several NCD mutants, and established that the stability loss of specific NCD mutants contributes to their reduced velocities and anomalous directionality on the microtubules. This provides a rare case where the origin of the AI prediction is clearly related to a physical-based picture.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.