ArticleAnalytical chemistry2026
Coupling Liquid Chromatography to Orbitrap Isotope Ratio Mass Spectrometry: Overcoming Isotope Effects of Chromatography and Amount-Dependency by Peak Homogenization.
Article in Analytical chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
2 citing papers in PubMed.
- Determination of stable carbon isotope ratios for molecules in natural organic matter using ESI FT-ICR MS.Science advances · 2026Article
- Intramolecularly Labeled Reference Standards of Sulfamethoxazole for Fragment-Specific Isotope Analysis by Electrospray Ionization Orbitrap Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2026Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Electrospray ionization Orbitrap mass spectrometry (ESI-Orbitrap-MS) has recently proven to be a powerful tool for compound- and position-specific stable isotope analysis, targeting isotopologues of multiple elements (H, C, N, O, S) in polar analytes. However, studies have so far mainly focused on pure analyte solutions via direct infusion. Here, we present the online coupling of liquid chromatography (LC) to ESI-Orbitrap-MS for stable isotope analysis of sulfamethoxazole (SMX), a synthetic antimicrobial, used as a model compound. Our study explored the fidelity of isotope values with two strategies for capturing and broadening chromatographic peaks after LC. When capturing the target analyte in a capillary (0.7 mm inner diameter), peak shape was retained, resulting in systematic deviations in isotope values caused by isotope effects from chromatography of up to 70‰ and amount-dependency of up to 65‰. A dynamic mixing chamber was used to homogenize the target analyte upon elution, removing these deviations and resulting in stable carbon, nitrogen, and sulfur isotopologue ratios in various fragments of sulfamethoxazole across the chromatographic peak. Comparison with data from conventional magnetic sector isotope ratio mass spectrometry successfully calibrated the setup for carbon and sulfur isotope ratio analysis. This resulted in a precision for δ
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Registered trials
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