ArticleScientific reports2025
Functional and proteomic analysis of Lactobacillus rhamnosus-derived extracellular vesicles with antioxidant and anti-inflammatory activity.
Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- Postbiotics as Multifunctional Bioactives: Mechanistic Insights and Translational Applications in Host Physiology and Microbial Ecosystem Modulation.Microorganisms · 2026Review
- Reprogramming the gut-liver immune axis: probiotic-derived extracellular vesicles as precision nanotherapeutics in hepatocellular carcinoma.Infectious agents and cancer · 2026Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
Abstract
Extracellular vesicles (EVs) derived from lactic acid bacteria have gained attention as mediators of host-microbe interaction. However, their molecular characteristics compared to cell-derived vesicles (CDVs) remain poorly understood. In this study, we isolated EVs and CDVs from Lactobacillus rhamnosus and characterized them throughout proteomic and functional analyses. Both vesicle types displayed spherical morphology as observed by nanoparticle tracking analysis and transmission electron microscopy. Western blotting revealed distinct protein profiles, with HSP70 specifically detected in EVs. Proteomic profiling using LC-MS/MS identified a total of 1,342 proteins, including 114 unique to CDVs. Differential expression proteins analysis showed 70 proteins upregulated and 81 downregulated in EVs. Gene Ontology (GO) analysis indicated that EVs were enriched in proteins related to signal transduction, environmental adaptation, and transmembrane transport, while CDVs showed enrichment in ribosomal and nucleic acid metabolism-related proteins. Functional assays further demonstrated that EVs exhibited significantly stronger antioxidant and anti-inflammatory activities than CDVs. These findings suggest that L. rhamnosus-derived EVs possess distinct proteomic signatures and superior bioactivity, supporting their potential role in probiotic-based therapeutic strategies, particularly for skin health.
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Registered trials
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