ArticleScientific reports2025
In vitro and in silico evaluation of flavonoids from Erythrina crista-galli with cytotoxic potential against MCF-7 breast cancer cell.
Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Naturally occurring flavonoids have garnered significant interest as potential anticancer agents owing to their structural diversity and ability to modulate multiple molecular targets. This study aimed to investigate the cytotoxic potential of flavonoids isolated from Erythrina crista-galli in MCF-7 breast cancer cells using in vitro and in silico analyses. Three flavonoids were isolated from the twigs of E. crista-galli using multiple column chromatography. Cytotoxic activity was evaluated against the MCF-7 breast cancer cell line using the MTT assay, whereas the in silico study was evaluated using molecular docking and molecular dynamics against the epidermal growth factor receptor (EGFR). Three flavonoids, naringenin (1), daidzein (2), and isoliquiritigenin (3), were isolated from the ethanol extract of E. crista-galli twigs. Their structures were confirmed using multiple spectroscopic methods. In vitro cytotoxicity testing against MCF-7 breast cancer cells showed that naringenin (1) and isoliquiritigenin (3) exhibited moderate activity, with IC50 values of 105.60 and 84.21 µg/mL, respectively, whereas daidzein (2) displayed weak activity, with IC50 of 239.77 µg/mL. Network pharmacology analysis identified multiple targets of isoliquiritigenin related to breast cancer, with epidermal growth factor receptor (EGFR) as one of the key hub. Molecular docking indicated a stronger binding affinity of isoliquiritigenin compared to ATP, while molecular dynamics simulations confirmed the stability of the EGFR–isoliquiritigenin complex with binding free energy estimated at − 30.002 ± 3.879 kcal/mol. Collectively, these results highlight isoliquiritigenin as a promising EGFR-targeted cytotoxic compound and E. crista-galli as a valuable source of bioactive flavonoids for anticancer drug development, while emphasizing the need for further in vivo validation and toxicity assessment.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.