An optimized sandwich ELISA for quantitative detection of fowl adenovirus serotype 4 (FAdV-4) in chickens.

Huimin Ma, Yuhang Zhou, Shipeng Wang, Xiangyu Xie, Ruiji Chen, Qi Zheng, Lisha Zha, Xinyue Chang

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Article in Poultry science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

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8 authors.

Huimin MaCollege of Veterinary Medicine, Anhui Agricultural University, Hefei 230036, PR China.

Yuhang ZhouCollege of Veterinary Medicine, Anhui Agricultural University, Hefei 230036, PR China.

Shipeng WangCollege of Veterinary Medicine, Anhui Agricultural University, Hefei 230036, PR China.

Xiangyu XieCollege of Veterinary Medicine, Anhui Agricultural University, Hefei 230036, PR China.

Ruiji ChenCollege of Veterinary Medicine, Anhui Agricultural University, Hefei 230036, PR China.

Qi ZhengCollege of Veterinary Medicine, Anhui Agricultural University, Hefei 230036, PR China.

Lisha ZhaSchool of Biomedical Sciences, Hunan University, Changsha 410082, PR China.

Xinyue ChangCollege of Veterinary Medicine, Anhui Agricultural University, Hefei 230036, PR China. Electronic address: xinyuechang@ahau.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Fowl adenovirus serotype 4 (FAdV-4) causes hydropericardium-hepatitis syndrome (HHS) in chickens, resulting in severe economic losses worldwide. Fast and accurate diagnostic methods for FAdV-4 are required to monitor its prevalence and provide plausible strategies for prevention. Sandwich ELISA is a common and rapid diagnostic technique; however, an FAdV-4 ELISA kit is not commercially available in China. Fiber 1 protein of FAdV-4 has been used as diagnostic targets. In this study, we firstly screened 5 monoclonal antibodies (mAbs) against fiber 1 from rabbits using phage display. ELISA and indirect fluorescence assay (IFA) showed these mAbs specifically bound to both fiber 1 and FAdV-4. Binding affinities of the mAbs forming optimal pair were lower than 10

Indexed as

Adenoviridae InfectionsAviadenovirusChickensPoultry DiseasesAnimalsChinaEnzyme-Linked Immunosorbent AssayReproducibility of ResultsSensitivity and SpecificitySerogroupFAdV-4Fiber 1 proteinMonoclonal antibody (mAb)Sandwich ELISA

Identifiers

PMID41411855

DOI10.1016/j.psj.2025.106259

PMCPMC12950391

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.