Evidence map›Paper›PMID 41411132›Full record

ArticleCell reports2026

Endothelial cell stiffness and type drive the formation of biomechanically induced transcellular pores.

Seyed Mohammad Siadat, Haiyan Li, Brigid Millette, Babak N Safa, Cydney A Wong, M Reza Bahrani Fard, Sietse T Braakman, Ian Tay, Jacques A Bertrand, A Thomas Read and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Novel Role for CGRP in Aqueous Humor Outflow.Investigative ophthalmology & visual science · 2026
    Article
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

15 authors.

Seyed Mohammad SiadatWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology/Emory University, Atlanta, GA, USA; Department of Bioengineering, Northeastern University, Boston, MA, USA.
Haiyan LiWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology/Emory University, Atlanta, GA, USA.
Brigid MilletteWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology/Emory University, Atlanta, GA, USA.
Babak N SafaWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology/Emory University, Atlanta, GA, USA; Department of Medical Engineering, University of South Florida, Tampa, FL, USA.
Cydney A WongWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology/Emory University, Atlanta, GA, USA.
M Reza Bahrani FardWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology/Emory University, Atlanta, GA, USA.
Sietse T BraakmanDepartment of Bioengineering, Imperial College London, London, UK.
Ian TayDepartment of Bioengineering, Imperial College London, London, UK.
Jacques A BertrandDepartment of Bioengineering, Imperial College London, London, UK.
A Thomas ReadWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology/Emory University, Atlanta, GA, USA.
Lisa SchildmeyerWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology/Emory University, Atlanta, GA, USA.
Kristin M PerkumasDepartment of Ophthalmology, Duke Eye Center, Duke University, Durham, NC, USA.
W Daniel StamerDepartment of Ophthalmology, Duke Eye Center, Duke University, Durham, NC, USA.
Darryl R OverbyDepartment of Bioengineering, Imperial College London, London, UK.
C Ross EthierWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology/Emory University, Atlanta, GA, USA. Electronic address: ross.ethier@emory.edu.

Funding

eNOS-Dependent Mechanoregulation of Intraocular PressureR01EY022359 · NEI · DUKE UNIVERSITY · PI STAMER, W DANIEL · 2012 to 2025
$5.6M
Caveolae-Based Mechanosensors for Conventional Outflow RegulationR01EY028608 · NEI · UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR · PI MICHAEL H ELLIOTT, W Daniel Stamer · 2018 to 2026
$3.8M
Transcriptional and Biomechanical Analysis of Segmental Outflow in GlaucomaR01EY031710 · NEI · GEORGIA INSTITUTE OF TECHNOLOGY · PI ETHIER, C ROSS, GUPTA, DIVAKAR · 2020 to 2023
$2.4M
T32 CTEng (Cellular and Tissue Engineering) Training ProgramT32GM145735 · NIGMS · GEORGIA INSTITUTE OF TECHNOLOGY · PI Edward A. Botchwey, Andres J Garcia · 2022 to 2026
$2.3M
The role of iris biomechanics and sex differences in primary angle-closure glaucomaR00EY035360 · NEI · UNIVERSITY OF SOUTH FLORIDA · PI Babak Naghizadehsafa · 2024 to 2026
$747k
Schlemm’s canal on a chip: A platform for screening a novel class of glaucoma medicationsR21EY033142 · NEI · GEORGIA INSTITUTE OF TECHNOLOGY · PI ETHIER, C ROSS · 2021 to 2022
$405k
The role of iris biomechanics and sex differences in primary angle-closure glaucomaK99EY035360 · NEI · GEORGIA INSTITUTE OF TECHNOLOGY · PI NAGHIZADEH SAFA, BABAK · 2023 to 2024
$224k
NEI NIH HHS K99 EY035360NEI NIH HHS R00 EY035360NEI NIH HHS R01 EY022359NEI NIH HHS R01 EY028608NEI NIH HHS R01 EY031710NEI NIH HHS R21 EY033142NIGMS NIH HHS T32 GM145735
6 · The paper itself

Abstract

Formation of transcellular pores facilitates material transport across endothelial barriers. In Schlemm's canal (SC) endothelium, impaired pore formation is linked to glaucoma, but the underlying processes remain poorly understood due to a lack of in vitro assays. Here, we present a platform to study pore formation in human endothelial cells. Pores were induced in SC cells by seeding them atop micron-sized magnetic beads and applying a magnetic field to generate basal-to-apical forces, mimicking in vivo biomechanics. Pore formation was dynamic, with pores opening and closing. Glaucomatous cells showed impaired pore formation, which correlated with increased stiffness. Notably, apical-to-basal forces failed to induce pores in SC cells but triggered pore formation in other endothelial types. These findings highlight the central role of cell mechanics in transcellular pore formation and provide an approach to investigate the mechanisms underlying this process.

Indexed as

Endothelial CellsTranscytosisBiomechanical PhenomenaGlaucomaHumansaqueous humor outflowbiomechanicscell stiffnessCP: cell biologyendothelial cellsglaucomaintraocular pressure regulationmechanobiologymechanotransductionSchlemm’s canaltranscellular pores

Identifiers

PMID41411132
PMCPMC12951807

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.