Evidence map›Paper›PMID 41410178›Full record

ArticleAngewandte Chemie (International ed. in English)2026

Intact Proteoform Analysis by Capillary Electrophoresis-Mass Spectrometry. Are We There Yet?

Noah Gould, Qianjie Wang, Jeffrey N Agar, Jennifer S Brodbelt, Daoyang Chen, Kellye A Cupp-Sutton, Elena Domínguez-Vega, Fei Fang, Marianne Fillet, Matthew S Fischer and 24 more

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In one paragraph

Article in Angewandte Chemie (International ed. in English), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Review
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

34 authors.

Noah Gould *Barnett Institute of Chemical and Biological Analysis and Department of Chemistry & Chemical Biology, Northeastern University, Boston, MA, USA.ORCID 0000-0002-6664-703X
Qianjie Wang *Department of Chemistry, Michigan State University, East Lansing, MI, USA.
Jeffrey N AgarBarnett Institute of Chemical and Biological Analysis and Department of Chemistry & Chemical Biology, Northeastern University, Boston, MA, USA.
Jennifer S BrodbeltDepartment of Chemistry, University of Texas, Austin, TX, USA.
Daoyang ChenDiscovery Biologics, Merck Research Laboratory, USA.
Kellye A Cupp-SuttonDepartment of Chemistry and Biochemistry, University of Alabama, Tuscaloosa, AL, USA.
Elena Domínguez-VegaCenter for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, The Netherlands.
Fei FangDepartment of Chemistry, Michigan State University, East Lansing, MI, USA.
Marianne FilletLaboratory for the Analysis of Medicines, Center for Interdisciplinary Research on Medicines (CIRM), University of Liege, Quartier Hospital, Liege, Belgium.
Matthew S FischerDepartment of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin, 53706, USA.
Attila GasparDepartment of Inorganic and Analytical Chemistry, University of Debrecen, Debrecen, Hungary.
Ying GeDepartment of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin, 53706, USA.
Marie-Jia GouLaboratory for the Analysis of Medicines, Center for Interdisciplinary Research on Medicines (CIRM), University of Liege, Quartier Hospital, Liege, Belgium.
Michal GregušBarnett Institute of Chemical and Biological Analysis and Department of Chemistry & Chemical Biology, Northeastern University, Boston, MA, USA.
Christoph GstöttnerCenter for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, The Netherlands.
Narmin HamidliDepartment of Inorganic and Analytical Chemistry, University of Debrecen, Debrecen, Hungary.
Amanda HelmsDepartment of Chemistry, University of Texas, Austin, TX, USA.
Md Amin HossainBarnett Institute of Chemical and Biological Analysis and Department of Chemistry & Chemical Biology, Northeastern University, Boston, MA, USA.
Kyle J JuettenDepartment of Chemistry, University of Texas, Austin, TX, USA.
Neil L KelleherDepartments of Chemistry and Molecular Biosciences, the Chemistry of Life Processes Institute, and the Proteomics Center of Excellence, Northwestern University, Evanston, IL, USA.
Tobias KrausDepartment of Chemistry, Aalen University, Aalen, Germany.
Eli J LarsonDepartment of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin, 53706, USA.
J Scott Mellors908 Devices Inc., Boston, MA, USA.
Cynthia NagyDepartment of Inorganic and Analytical Chemistry, University of Debrecen, Debrecen, Hungary.
Christian NeusüßDepartment of Chemistry, Aalen University, Aalen, Germany.
Erin A Redman908 Devices Inc., Boston, MA, USA.
Jasmin SchairerDepartment of Chemistry, Aalen University, Aalen, Germany.
Si WuDepartment of Chemistry and Biochemistry, University of Alabama, Tuscaloosa, AL, USA.
Tian XuDepartments of Chemistry and Molecular Biosciences, the Chemistry of Life Processes Institute, and the Proteomics Center of Excellence, Northwestern University, Evanston, IL, USA.
Zhitao ZhaoDepartment of Chemistry and Biochemistry, University of Alabama, Tuscaloosa, AL, USA.
Guijie ZhuDepartment of Chemistry, Michigan State University, East Lansing, MI, USA.
Alexander R Ivanov *Barnett Institute of Chemical and Biological Analysis and Department of Chemistry & Chemical Biology, Northeastern University, Boston, MA, USA.ORCID 0000-0002-4691-8488
Kevin Jooß *Division of Bioanalytical Chemistry, Department of Chemistry and Pharmaceutical Sciences, Amsterdam Institute of Molecular and Life Sciences, Vrije Universiteit Amsterdam, Centre for Analytical Sciences Amsterdam (CASA), Amsterdam, The Netherlands.
Liangliang Sun *Department of Chemistry, Michigan State University, East Lansing, MI, USA.

Funding

Translation of immunologic technologies from basic research into pre-clinical nonU19AI062629 · NIAID · OKLAHOMA MEDICAL RESEARCH FOUNDATION · PI COGGESHALL, KENNETH MARK · 2004 to 2023
$56.2M
Waters Select Series Cyclic IMS for P41 Native MS Resource Application to Alzheimer's DiseaseP41GM128577 · NIGMS · OHIO STATE UNIVERSITY · PI WYSOCKI, VICKI H. · 2018 to 2022
$7.6M
Effect of methodological and biological variability on molecular profiling of extracellular vesicles in cancer detectionR01CA218500 · NCI · NORTHEASTERN UNIVERSITY · PI DEL MONTE, FEDERICA, GHIRAN, IONITA CALIN · 2018 to 2022
$4.0M
Deciphering Myofilament Modifications in Ischemic CardiomyopathyR01HL109810 · NHLBI · UNIVERSITY OF WISCONSIN-MADISON · PI GE, YING · 2013 to 2024
$3.5M
Robust ultra-high sensitivity proteomic technologies for limited samplesR35GM136421 · NIGMS · NORTHEASTERN UNIVERSITY · PI Alexander R. Ivanov · 2020 to 2026
$3.4M
Ultraviolet Photodissociation Mass Spectrometry for Characterization of Biological MoleculesR35GM139658 · NIGMS · UNIVERSITY OF TEXAS AT AUSTIN · PI BRODBELT, JENNIFER S. · 2021 to 2025
$2.9M
Enabling Top-Down Proteomics through Materials Chemistry and NanotechnologyR01GM117058 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI GE, YING, JIN, SONG · 2015 to 2023
$2.7M
Quantitative Analysis of Serum Autoantibody Repertories in Systemic Lupus ErythematosusR01AI141625 · NIAID · UNIVERSITY OF OKLAHOMA · PI SINGH, SHANTERI, SMITH, KENNETH MICHAEL · 2019 to 2023
$2.1M
Quantitative top-down proteomics of human colorectal cancer cells and tumorsR01CA247863 · NCI · MICHIGAN STATE UNIVERSITY · PI HUMMON, AMANDA B., LIU, XIAOWEN · 2021 to 2025
$1.9M
Advancing top-down proteomics with capillary electrophoresis-mass spectrometryR35GM153479 · NIGMS · MICHIGAN STATE UNIVERSITY · PI Liangliang Sun · 2024 to 2026
$1.4M
Next-generation nanoflow LC column technology to enable high sensitivity proteomics of limited samplesR41GM156145 · NIGMS · MIXEDLCMEDIA LLC · PI IVANOV, ALEXANDER R · 2024 to 2024
$307k
National Institute of Allergy and Infectious Diseases 2U19AI062629National Institute of Allergy and Infectious Diseases R01AI141625NCI NIH HHS R01 CA218500NCI NIH HHS R01 CA247863NCI NIH HHS R01CA247863Nemzeti Kutatási Fejlesztési és Innovációs Hivatal K124134NHLBI NIH HHS R01 HL109810NHLBI NIH HHS R01HL109810NIAID NIH HHS R01 AI141625NIAID NIH HHS U19 AI062629NIGMS NIH HHS 5P41GM128577NIGMS NIH HHS P41 GM128577NIGMS NIH HHS R01 GM117058NIGMS NIH HHS R01GM117058NIGMS NIH HHS R35 GM136421NIGMS NIH HHS R35 GM139658NIGMS NIH HHS R35 GM153479NIGMS NIH HHS R41 GM156145NIGMS NIH HHS R41GM156145Oklahoma Center for the Advancement of Science and Technology HR23-169Universiteit Leiden LUMC Fellowship (2020)
6 · The paper itself

Abstract

Mass spectrometry (MS)-based top-down proteomics (TDP) has emerged as a powerful tool for characterizing proteoforms to advance both fundamental and translational research. TDP requires high-efficiency liquid-phase separation, high-resolution MS, and tandem MS. Capillary zone electrophoresis (CZE)-MS has been proposed as a promising analytical technique for protein analysis decades ago because of its unique and valuable features, including high separation efficiency and high detection sensitivity. However, CZE-MS has not been widely adopted by the proteomics community, mainly due to concerns with its robustness and reproducibility. Here, we hypothesized that CZE-MS is sufficiently robust and reproducible for broad adoption due to the continued efforts of the community over the last three decades. In this work, for the first time, research teams from around the world validated the robustness, repeatability, and reproducibility of CZE-MS for TDP in both simple and complex model proteoform mixtures employing a full spectrum of commercially available capillary electrophoresis (CE)-MS interfaces, instrumentation, and compared CZE-MS performance with state-of-the-art liquid chromatography (LC)-MS methods. This study offers the research community an informative resource of ready-to-use experimental CE-MS techniques and a better understanding of the CZE-MS approach and its potential in TDP, accelerating the broad adoption of CZE-MS in proteoform research.

Indexed as

Mass SpectrometryProteinsProteomicsElectrophoresis, CapillaryProteinsAnalytical methodsElectrophoresisMass spectrometryProteomics

Identifiers

PMID41410178
PMCPMC12814393

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.