ArticleThe Journal of veterinary medical science2026
Immunophenotypic characterization of canine oligodendroglioma cells cultured in an oligodendrocyte precursor cell-tailored medium.
Article in The Journal of veterinary medical science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Canine oligodendrogliomas express platelet-derived growth factor receptor alpha (PDGFRA), an oligodendrocyte precursor cell (OPC) marker. In this study, tumor cells derived from 3 French bulldogs with oligodendroglioma were cultured in conventional medium with 10% fetal bovine serum (FBS) and an OPC-tailored medium containing platelet-derived growth factor (PDGF)-AA and basic fibroblast growth factor (bFGF). Immunocytochemically, cells cultured with 10% FBS were negative for PDGFRA, but tended to express 2',3'-cyclicnucleotide 3'-phosphodiesterase (CNPase) and β3-tubulin. In contrast, the cells cultured with PDGF-AA and bFGF tended to express PDGFRA. These results suggest that canine oligodendroglioma cells cultured with PDGF-AA and bFGF exhibit OPC-like immunophenotype. Cells cultured with OPC-tailored medium may be useful for further in vitro research on canine oligodendrogliomas.
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