Evidence map›Paper›PMID 41406966›Full record

ArticleCurrent biology : CB2026

Pth4 neurons define a novel hypothalamic circuit that promotes sleep via brainstem monoaminergic neurons.

Ulrich Herget, Steven Tran, Chanpreet Singh, Grigorios Oikonomou, Soojin Ryu, Josep Rotllant, David A Prober

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Current biology : CB, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors.

Ulrich HergetTianqiao and Chrissy Chen Institute for Neuroscience, Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
Steven TranTianqiao and Chrissy Chen Institute for Neuroscience, Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA; Department of Psychology, California State University, Fullerton, Fullerton, CA 92831, USA.
Chanpreet SinghTianqiao and Chrissy Chen Institute for Neuroscience, Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
Grigorios OikonomouTianqiao and Chrissy Chen Institute for Neuroscience, Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
Soojin RyuLiving Systems Institute, Department of Clinical and Biomedical Sciences, University of Exeter, Exeter EX4 4QD, UK.
Josep RotllantDepartment of Biotechnology and Aquaculture, Institute of Marine Research, 36208 Vigo, Spain.
David A ProberTianqiao and Chrissy Chen Institute for Neuroscience, Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA. Electronic address: dprober@caltech.edu.

Funding

Genetic and Neuronal Mechanisms that Regulate Zebrafish Sleep - DIVERSITY SUPPLEMENTR35NS122172 · NINDS · CALIFORNIA INSTITUTE OF TECHNOLOGY · PI David Aaron Prober · 2021 to 2026
$6.1M
NINDS NIH HHS R35 NS122172
6 · The paper itself

Abstract

Classical studies identified a critical role for the hypothalamus in regulating sleep and wake states, but few such hypothalamic neuronal populations have been identified. Here, we describe a sleep-promoting population of hypothalamic neurons that expresses the neuropeptides QRFP and parathyroid hormone 4 (Pth4) in zebrafish. Optogenetic stimulation of these neurons results in a large increase in sleep that requires pth4 but not qrfp. Noradrenergic locus coeruleus (LC) neurons and serotonergic (5HT) raphe nuclei (RNs) in the hindbrain express distinct pth receptors, and genetic epistasis and cell ablation experiments revealed that Pth4 neuron-induced sleep is suppressed in mutants that lack noradrenaline in the LC or lack the 5HT RNs. Pth4 neuron-induced sleep is also suppressed in serine/threonine kinase 32a (stk32a) mutants, possibly via stk32a-expressing neurons in the prethalamus that express pth receptors. These results identify QRFP/Pth4 neurons as a novel hypothalamic sleep-promoting population and support a model in which distinct sleep- and wake-promoting hypothalamic populations act via monoaminergic neurons in the hindbrain to control vigilance state.

Indexed as

Brain StemHypothalamusNeuronsParathyroid HormoneSleepZebrafishZebrafish ProteinsAnimalsParathyroid HormoneZebrafish Proteinsarousallocus coeruleusPth4QRFPraphesleepstk32azebrafish

Identifiers

PMID41406966
PMCPMC12818467

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.