Evidence map›Paper›PMID 41405671›Full record

ArticleVeterinary research communications2025

Innovative essential oil formulations for in vitro inhibition of Biofilm-Forming Extended-Spectrum β-Lactamase-Producing Escherichia coli isolated from canine infectious diarrhea.

Orathai Thongjuy, Sookruetai Boonmasawai, Sivapong Sungpradit, Phingphol Charoonrut, Kunaporn Homyog, Pathomporn Sresuwadjarey, Tipayawadee Jooypan, Pongsakorn Photcharatinnakorn, Supawan Nutakom, Arpron Leesombun

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Article in Veterinary research communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Orathai ThongjuyDepartment of Pre-Clinic and Applied Animal Science, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom, 73170, Thailand.
Sookruetai BoonmasawaiDepartment of Pre-Clinic and Applied Animal Science, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom, 73170, Thailand.
Sivapong SungpraditDepartment of Pre-Clinic and Applied Animal Science, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom, 73170, Thailand.
Phingphol CharoonrutDepartment of Clinical Science and Public Health, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom, 73170, Thailand.
Kunaporn HomyogCenter of Veterinary Diagnosis, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom, 73170, Thailand.
Pathomporn SresuwadjareyCenter of Veterinary Diagnosis, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom, 73170, Thailand.
Tipayawadee JooypanPrasu Arthorn Veterinary Teaching Hospital, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom, 73170, Thailand.
Pongsakorn PhotcharatinnakornPrasu Arthorn Veterinary Teaching Hospital, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom, 73170, Thailand.
Supawan NutakomPrasu Arthorn Veterinary Teaching Hospital, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom, 73170, Thailand.
Arpron LeesombunDepartment of Pre-Clinic and Applied Animal Science, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom, 73170, Thailand. arpron.lee@mahidol.ac.th.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe emergence of bacteria producing extended-spectrum β-lactamases (ESBLs) has presented a substantial challenge to veterinary and human medicine. ESBL-producing Escherichia coli exhibits biofilm-forming capabilities that enhance its persistence in domestic animals, contribute to chronic infectious diarrhea, and increase the risk of treatment failure. This study investigated ESBL-producing E. coli in dogs and evaluated the antimicrobial and antibiofilm activities of NHK-EO, an innovative essential oil formulation derived from Coleus amboinicus (N), Ocimum basilicum (H), and Ocimum tenuiflorum (K).

methodsThe chemical composition of NHK-EO was analyzed using gas chromatography-mass spectrometry (GC-MS). Fifty-six rectal swab samples were randomly collected from dogs aged 3 months to 10 years, including 30 clinically healthy and 26 diarrheic animals of both sexes. Bacterial isolation and identification were conducted using conventional biochemical tests and the VITEK automated system. ESBL production was confirmed through genotypic detection of bla

resultsGC-MS analysis of NHK-EO revealed three major constituents: carvacrol (16.37%), eugenol (15.04%), and linalool (13.97%). In total, 78 Gram-negative bacterial isolates were identified, with E. coli being the predominant species (56 isolates, 71.79%). Among these, four bla

conclusionsNHK-EO possesses significant antimicrobial and antibiofilm activities against E. coli, including ESBL-producing strains, indicating its potential as an alternative therapeutic agent to mitigate antimicrobial resistance. Further research is warranted to explore its clinical applications in veterinary and human medicine.

Indexed as

Anti-Bacterial AgentsBiofilmsDiarrheaDog DiseasesEscherichia coliEscherichia coli InfectionsOils, VolatileAnimalsbeta-LactamasesDogsFemaleMaleMicrobial Sensitivity TestsAnti-Bacterial Agentsbeta-LactamasesOils, VolatileBiofilm formationbla CTX−MCanine infectious diarrheaESBL-producing Escherichia coliEssential oil mixture

Identifiers

PMID41405671
PMCPMC12711979

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.