DNA-Guided Robust Single-Protein Electronic Readout.

Ziyi Ju, Zhaoxiang Deng, Yueqi Li, Jinghong Li

Abstract read

In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Ziyi JuHefei National Research Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, 230026, China.ORCID https://orcid.org/0009-0007-9073-7629

Zhaoxiang DengHefei National Research Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, 230026, China.ORCID https://orcid.org/0000-0003-0811-5147

Yueqi LiHefei National Research Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, 230026, China.ORCID https://orcid.org/0000-0001-7003-1978

Jinghong LiHefei National Research Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, 230026, China.ORCID https://orcid.org/0000-0002-0750-7352

Funding

National Natural Science Foundation of China 22434005

6 · The paper itself

Abstract

Electronic transport properties of single proteins offer powerful insights into their structure and dynamics. However, the orientation variability of electrode-attached protein molecules poses a big difficulty in achieving precise electric measurements and reliable data interpretation. Here, a DNA origami-based method that promotes preferred orientations of target proteins is reported, enabling more reproducible single-molecule electrical characterization. With two orthogonal DNA aptamers installed inside a DNA origami nanocavity, a thrombin protein can be captured with a prescribed orientation between a gold substrate and a conductive AFM tip. Matrix-patterned I-V measurements reveal that bivalently anchored thrombin molecules exhibit significantly reduced conductance variability compared to randomly adsorbed ones. This critical progress then enables the detection of a subtle conductance change of thrombin upon binding with Na

Indexed as

Biosensing TechniquesDNANanotechnologyThrombinAptamers, NucleotideGoldMicroscopy, Atomic ForceAptamers, NucleotideDNAGoldThrombinbiosensingDNA origamiprotein conductance measurementsingle‐molecular conductance

Identifiers

PMID41405386

DOI10.1002/advs.202516711

PMCPMC12948236

What OpenQuestion holds

Textmetadata

LicenceCC BY

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.