Engineering artificial 5' regulatory sequences for thermostable protein expression in the extremophile

Che Fai Alex Wong, Shizhe Zhang, Lisa Tietze, Gurvinder Singh Dahiya, Rahmi Lale

Abstract read

In one paragraph

Article in Synthetic biology (Oxford, England), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Che Fai Alex WongDepartment of Biotechnology and Food Science, Faculty of Natural Sciences, Norwegian University of Science and Technology, NO-7491, Trondheim, Norway.

Shizhe ZhangDepartment of Biotechnology and Food Science, Faculty of Natural Sciences, Norwegian University of Science and Technology, NO-7491, Trondheim, Norway.

Lisa TietzeDepartment of Biotechnology and Food Science, Faculty of Natural Sciences, Norwegian University of Science and Technology, NO-7491, Trondheim, Norway.

Gurvinder Singh DahiyaSyngens AS, NO-7089, Trondheim, Norway.

Rahmi LaleDepartment of Biotechnology and Food Science, Faculty of Natural Sciences, Norwegian University of Science and Technology, NO-7491, Trondheim, Norway.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The utilization of biocatalysts in biotechnological applications often necessitates their heterologous expression in suitable host organisms. However, the range of standardized microbial hosts for recombinant protein production remains limited, with most being mesophilic and suboptimal for certain protein types. Although the thermophilic bacterium

Indexed as

artificial promoterEscherichia coliextremophilesthermostable protein expressionThermus thermophilus

Identifiers

PMID41404420

DOI10.1093/synbio/ysaf016

PMCPMC12704435

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.