Evidence map›Paper›PMID 41404152›Full record

ArticleFrontiers in plant science2025

ROS-mediated membrane damage and antioxidant imbalance drive apple flesh browning during cold storage.

Jihan Wang, Fujun Li, Bingru Li, Ling Li, Jing Shang, Xiaodong Fu, Xiuming Zhao, Xiaoan Li, Xinhua Zhang, Zienab F R Ahmed

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in plant science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Jihan Wang *College of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, Shandong, China.
Fujun Li *College of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, Shandong, China.
Bingru LiCollege of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, Shandong, China.
Ling LiCollege of Food and Biological Engineering, Beijing Vocational College of Agriculture, Beijing, China.
Jing ShangCollege of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, Shandong, China.
Xiaodong FuCollege of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, Shandong, China.
Xiuming ZhaoCollege of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, Shandong, China.
Xiaoan LiCollege of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, Shandong, China.
Xinhua ZhangCollege of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, Shandong, China.
Zienab F R AhmedIntegrative Agriculture Department, College of Agriculture and Veterinary Medicine, United Arab Emirates University, Al Ain, United Arab Emirates.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Flesh browning is a major postharvest disorder in apples during cold storage, yet its physiological basis remains unclear. This study examined reactive oxygen species (ROS) metabolism and antioxidant systems in flesh-browning (FB) and normal (FN) tissues of browning apples, compared with healthy fruit (Control). Methods: 'Fuji' apples were stored at -1-0°C for 120 days. Flesh color, membrane damage indicators, phenolic metabolism, antioxidant contents (AsA, GSH), ROS levels (H Results: FB tissues showed the highest PPO and POD activities, enhanced membrane damage (higher EC, MDA, LOX), and markedly elevated ROS. Total antioxidant capacity decreased by ~25% in FB, accompanied by significant reductions in AsA and GSH and lower CAT/APX activities. Although phenylpropanoid-related enzymes and genes were upregulated, the resulting phenolics and flavonoids were insufficient for ROS mitigation. Strong correlations were found between browning intensity (a*), ROS accumulation, membrane damage, and antioxidant depletion. Discussion: Prolonged cold storage induces severe ROS accumulation and membrane disruption in FB tissues. Simultaneous reductions in non-enzymatic antioxidants (AsA/GSH) and antioxidant enzymes (CAT/APX) create a self-reinforcing imbalance in ROS metabolism, ultimately triggering enzymatic browning.

Indexed as

antioxidant imbalanceapple flesh browningcold storagecompartmentalization damageROS metabolism

Identifiers

PMID41404152
PMCPMC12703709

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.