ArticleCommunications biology2025
Biotin methyl ester enhances cargo release in RUSH system and enables rapid biotinylation with TurboID.
Article in Communications biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
2 citing papers in PubMed.
- Reversible control of post-Golgi transport by brefeldin A reveals recycling endosome maturation during glycosylphosphatidylinositol-anchored protein transport.Nature communications · 2026Article
- An engineered streptavidin condensate platform for chemically inducible control of endogenous proteins in mammalian cells.bioRxiv : the preprint server for biology · 2026Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Biotin-streptavidin technology is a key tool in biotechnology. The engineering of streptavidin or streptavidin-like molecules and organic synthesis of biotin derivatives offer a variety of applications to meet the needs of researchers. Here, we report that owing to its masked charged carboxyl group, biotin methyl ester (BME) improves cell penetration and allows the retention using selective hooks (RUSH) system to initiate cargo release promptly and uniformly in all cells. In addition, because of the rapid hydrolysis of BME to biotin inside cells, BME enabled faster biotinylation than biotin, by the promiscuous biotin ligase TurboID. These properties allow BME to act as a biotin prodrug, enhance the RUSH system and TurboID, and highlight the potential of biotin-streptavidin technology for numerous applications in living cells. Additionally, BME may be a good biotin supplement for patients with multivitamin-responsive inherited metabolic disorders.
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Registered trials
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