ArticleScientific reports2025
Physiological fluid shear stress synergistically enhances the protective effects of Salvia miltiorrhiza extract on endothelial cells.
Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- The Conventional and Alternative Therapeutic Approaches in Arterial Stiffness Management.Pharmaceutics · 2026Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
Natural medicines are gaining increasing attention in drug development, yet traditional static cell models fail to mimic the dynamic mechanical microenvironment in vivo, potentially leading to misleading evaluations. This study investigated how physiological fluid shear stress influences the protective effects of Salvia miltiorrhiza extract (SME) on endothelial cells (ECs). Using a parallel plate flow chamber, ECs were exposed to 15 dyn/cm² shear stress or static conditions, followed by H₂O₂-induced oxidative damage. Cell viability was assessed via MTT and live/dead staining; morphology and cytoskeleton were examined through phalloidin/DAPI staining; oxidative markers (ROS, SOD, MDA) were quantified; and SME bioactive component uptake was analyzed using HPLC. Under shear stress, SME's protection was significantly enhanced: metabolic viability reached 86.83 ± 6.4% (vs. 72.38 ± 6.8% static), protection rate nearly doubled (68.00% vs. 34.14%), morphology improved (shape index: 0.63 ± 0.11 vs. 0.73 ± 0.11), and oxidative damage was reduced (ROS decreased to 13.73 ± 1.47; SOD increased to 41.24 U/mg; MDA decreased to 0.74 nmol/mg). HPLC revealed enhanced absorption of all compounds and uptake of two additional bioactive constituents. These findings demonstrate that physiological shear stress potentiates SME's efficacy through improved compound delivery and amplified cellular responses, advocating for shear stress-responsive screening as a physiologically relevant strategy for natural medicine evaluation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.