ArticleScientific reports2025
Single-cell identification of an endothelial cell proximal SPP1
Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
3 citing papers in PubMed.
- 3D bioprinted human vascular organoid sheets promote functional ischemic repair and exhibit adaptive in vivo remodeling.BMC medicine · 2026Article
- Hemodynamics and matrix stiffness shape the pathogenicity of SPP1Frontiers in immunology · 2026Review
- Single-cell dissection of hepatocellular carcinoma immunity: from heterogeneous subtypes to precision therapeutics.Frontiers in immunology · 2026Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
Abstract
Head and neck squamous cell carcinoma (HNSCC) is a highly prevalent malignancy with poor prognosis, largely driven by lymph node metastasis (LNM). Despite its clinical significance, the underlying mechanisms of LNM remain elusive. In this study, we used single-cell transcriptomic data to dissect the cellular and molecular interactions within metastatic lymph nodes (MET). Specifically, we analyzed Single-cell RNA sequencing (scRNA-seq) data from GSE195655, GSE140042, GSE227156, and GSE159929 (n = 41) to delineate cellular heterogeneity, intercellular communication networks, and functional enrichment in primary tumors (PT), MET, and non-metastatic lymph nodes (LN). Our analysis identified a subpopulation of tumor-associated macrophages (TAMs) distinctly enriched in MET, characterized by high expression of SPP1. Functional analysis revealed that this TAM subpopulation promotes angiogenesis through specific ligand-receptor interactions with endothelial cells (ECs), involving the SPP1-ITGa9b1 and FN1-ITGa2b1 signaling axes. Furthermore, we leveraged bulk RNA-seq for prognostic research. Immunohistochemistry (IHC) confirmed the increased density and number of blood and lymphatic vessels in MET. Spatial analysis via multiplex immunohistochemistry (mIHC) confirmed the preferential localization of SPP1
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.