ArticleZhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics2025
[The miR-30b/USP14 axis regulates mitophagy via a non-PINK1/Parkin pathway in neuronal oxygen-glucose deprivation/reoxygenation injury].
Article in Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
objectivesTo investigate how miR-30b regulates mitophagy independently of the PINK1/Parkin pathway by targeting ubiquitin specific peptidase 14 (USP14) in neuronal oxygen-glucose deprivation/reoxygenation (OGD/R) injury, and to provide new insights for the treatment of neonatal hypoxic-ischemic encephalopathy.
methodsFetal rat cortical neurons were isolated and an OGD/R model was established. Experiments were conducted in two parts. In part 1, cells were randomized into control, OGD/R, OGD/R+microRNA (miR)-negative control (NC), OGD/R+miR-30b mimic, OGD/R+miR-30b mimic+oe-NC, and OGD/R+miR-30b mimic+oe-USP14 groups. In part 2, cells were randomized into control, OGD/R, OGD/R+miR-NC, OGD/R+miR-30b mimic, OGD/R+miR-30b mimic+si-NC, OGD/R+miR-30b mimic+si-Parkin, and OGD/R+miR-30b mimic+si-Drp1 (dynamin-related protein 1) groups. miR-30b and USP14 mRNA levels were measured by real-time quantitative PCR. Cell viability was assessed using the CCK-8 assay and cell death by propidium iodide staining. Protein levels of USP14, microtubule-associated protein 1 light chain 3 (LC3) Ⅱ/LC3 Ⅰ, translocase of outer mitochondrial membrane 20 (TOMM20), PINK1, Parkin, and Drp1 were determined by Western blot. MitoTracker Green staining was used to evaluate mitochondrial morphology, and mitochondrial reactive oxygen species (ROS) were measured using the MitoSOX Red probe. AGO
resultsCompared with the OGD/R group, the OGD/R+miR-30b mimic group showed higher cell viability, miR-30b expression, LC3 Ⅱ/LC3 Ⅰ ratio, and Drp1 protein expression (
conclusionsmiR-30b regulates mitophagy by targeting USP14 independently of the PINK1/Parkin pathway, and confers protection against neuronal OGD/R injury.
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