Evidence map›Paper›PMID 41400745›Full record

ArticleAnalytical sciences : the international journal of the Japan Society for Analytical Chemistry2026

Thermochemolysis-gas chromatographic detection of oxidized fatty acids in a single Drosophila melanogaster individual.

Yasuyuki Ishida, Misaki Haba, Noa Komoda, Nobuo Kido, Atsushi Watanabe

Abstract read
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In one paragraph

Article in Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Yasuyuki IshidaCollege of Bioscience and Biotechnology, Chubu University, 1200 Matsumoto-cho, Kasugai-shi, Aichi, 487-8501, Japan. yishida@fsc.chubu.ac.jp.
Misaki HabaCollege of Bioscience and Biotechnology, Chubu University, 1200 Matsumoto-cho, Kasugai-shi, Aichi, 487-8501, Japan.
Noa KomodaCollege of Bioscience and Biotechnology, Chubu University, 1200 Matsumoto-cho, Kasugai-shi, Aichi, 487-8501, Japan.
Nobuo KidoFaculty of Human Health and Science, Matsumoto University, 2095-1, Niimura, Matsumoto, Nagano, 390-1295, Japan.
Atsushi WatanabeFrontier Laboratories Ltd., Koriyama, Fukushima, 963-8862, Japan.

Funding

Chubu University Grant (A)Japan Society for the Promotion of Science 22K11765
6 · The paper itself

Abstract

Thermochemolysis-gas chromatography-mass spectrometry (Thermochemolysis-GC-MS) was applied to detect fatty acid oxidation products in a single Drosophila melanogaster individual (dry weight: 100-400 µg). Linoleic acid oxidized under UV irradiation was first analyzed to identify characteristic oxidation products, including azelaic acid. Using tetramethylammonium hydroxide (TMAH) as an organic alkali reagent, azelaic acid was successfully detected as its dimethyl ester in D. melanogaster individuals reared on linoleic acid-supplemented diets. The use of m/z 185 as a diagnostic ion in extracted ion chromatograms allowed for sensitive determination of azelaic acid in control and linoleic acid-treated D. melanogaster individuals. The treated groups exhibited notable increases in azelaic acid content, which demonstrates the method's ability to evaluate statistically significant differences based on individual-level data.

Indexed as

Drosophila melanogasterFatty AcidsGas Chromatography-Mass SpectrometryTemperatureAnimalsDicarboxylic AcidsLinoleic AcidOxidation-ReductionQuaternary Ammonium Compoundsazelaic acidDicarboxylic AcidsFatty AcidsLinoleic AcidQuaternary Ammonium CompoundsDrosophila melanogasterFatty acidOrganic alkaliOxidationThermochemolysis-GC–MS

Identifiers

PMID41400745

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.