Evidence map›Paper›PMID 41400516›Full record

ArticleBiology of reproduction2026

Long-term tamoxifen impacts the ovary but not preimplantation embryo development†.

Gülbahar Zehra Kutlutürk, Trenton L Place, Michael S Awadalla, Rachel B Danis, Sue Ann Ingles, Isaac Asante, Rita Li, Stan Louie, Frank Z Stanczyk, Lynda K McGinnis

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In one paragraph

Article in Biology of reproduction, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Gülbahar Zehra KutlutürkMolecular Pathology and Experimental Medicine, University of Southern California, Los Angeles, California, USA.
Trenton L PlaceReproductive Endocrinology and Infertility, University of Southern California, Los Angeles, California, USA.
Michael S AwadallaReproductive Endocrinology and Infertility, University of Southern California, Los Angeles, California, USA.
Rachel B DanisReproductive Endocrinology and Infertility, University of Southern California, Los Angeles, California, USA.
Sue Ann InglesPopulation and Public Health Sciences, University of Southern California, Los Angeles, California, USA.ORCID 0000-0001-9181-8745
Isaac AsanteOphthalmology, ITEMS, University of Southern California, Los Angeles, California, USA.ORCID 0000-0002-9628-3425
Rita LiClinical Pharmacy, University of Southern California, Los Angeles, California, USA.
Stan LouieOphthalmology, ITEMS, University of Southern California, Los Angeles, California, USA.ORCID 0000-0001-5941-6690
Frank Z StanczykReproductive Endocrinology and Infertility, University of Southern California, Los Angeles, California, USA.ORCID 0000-0002-3607-121X
Lynda K McGinnisReproductive Endocrinology and Infertility, University of Southern California, Los Angeles, California, USA.ORCID 0000-0001-7910-9655

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Many studies have examined the acute effects of chemotherapies and radiation on the ovary; however, few address the impact of long-term chemotherapeutic agents on future reproduction for cancer survivors. The purpose of our study was to determine the effect of long-term tamoxifen (TAM) treatment on follicle development, ovarian reserve, and embryogenesis in a murine model. Adult female mice were treated with TAM (250 mg/kg fed ad libitum in mouse chow) or control chow for three weeks, followed by 0-, 1-, or 3-weeks of TAM washout. Mice were then superovulated, mated, and their oviducts flushed to determine ovulation, fertilization, and in vitro embryo development rates. At specific timepoints, one ovary from each animal was harvested, sectioned, and stained with H&E, while the other ovary was evaluated for gene expression of targeted genes. Ovarian size was calculated based on average surface area over six slices. Follicles (primordial, primary, secondary, antral / pre-ovulatory), and corpora lutea (CL) were counted and averaged for each slice. All data were analyzed using mixed-effects analysis of variance (ANOVA), with treatment group as the fixed effect and ovary treated as a random effect nested within treatment. Ovaries of TAM treated mice were 36% smaller on average than control ovaries (P = 0.01). Primordial follicle counts were 35% lower than control ovaries (P = 0.007) and there were 3.4x more atretic follicles in the ovaries of TAM treated mice (P < 0.001). There were no significant differences in the number of primary, secondary, antral or pre-ovulatory follicles. The number of oocytes and embryos flushed from control mice (total ovulated) was 1.65x greater than TAM treated mice. However, fertilization rates and in vitro embryo blastulation rates were not significantly different between controls and TAM groups. In summary, for this murine model, long-term TAM negatively impacted ovarian reserve (primordial follicle number) without overtly affecting the in vitro embryo development to the blastocyst.

Indexed as

BlastocystEmbryonic DevelopmentOvaryTamoxifenAnimalsFemaleMiceOvarian FollicleOvarian ReserveTamoxifenblastocystesr2gperovaryovulationtamoxifen

Identifiers

PMID41400516
PMCPMC13016927

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