ArticleCurrent protocols2025
Rice Straw Tissue Preparation for Reproducible Electron Microscopy Imaging and Analysis.
Article in Current protocols, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- Rice straw sample preparation protocol for cell wall major constituent analysis via Raman spectroscopy.STAR protocols · 2026Article
- Rice Straw Tissue Preparation for Reproducible Electron Microscopy Imaging and Analysis.Current protocols · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Common problems in biological sample processing for scanning electron microscopy (SEM) include cell collapse and destruction. To overcome the challenges surrounding SEM micrograph preparation, dried rice stems were used to develop a specific set of protocols for processing dried plant samples. Dried stems are rehydrated with a glycerol solution and fixed in formalin-acetic-alcohol to avoid cell wall collapse or organ distortion. The protocols detailed here comprise the first published method for preparing SEM images of dried plant tissue. The protocols offer a cost-effective approach to obtaining high-quality micrographs, facilitating the reconstruction of growth processes and the study of plant cell wall features. © 2025 The Author(s). Current Protocols published by Wiley Periodicals LLC. Basic Protocol 1: Pretreatment and preparation of rice straw samples at the heading stage Basic Protocol 2: Paraffin infiltration and embedding Basic Protocol 3: Preparation of microscopic sections Basic Protocol 4: Transferring, adhering, and expanding sections on slides Support Protocol: Preparation of gelatin slides before sectioning to affix samples Basic Protocol 5: Preparation of samples for SEM imaging Basic Protocol 6: SEM analysis Basic Protocol 7: Processing and analysis of SEM images using ImageJ software.
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Registered trials
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