ArticleNature communications2025
Mechanism of alternative splicing of yeast HEH1 through competing 5' splice sites.
Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Alternative splicing of precursor-messenger RNAs (pre-mRNA) bearing introns with competing 5' splice sites (5'SS) produces two mRNAs. We investigated the mechanism of alternative splicing of the yeast HEH1/SRC1 gene, which contains four nucleotide-apart competing 5'SS, by monitoring its RNA and protein products. HEH1 alternative splicing requires a sixteen-nucleotide pre-mRNA segment spanning its two 5'SS. The nucleotides are decoded by U5 and U6 small nuclear RNAs (snRNAs), supported by specific proteins of the spliceosomal B and Bact complexes, including Prp8. HEH1 alternative splicing became independent of the supporting proteins following recalibration of the pre-mRNA-snRNA base pairings through changes in the 5'SS or U5 and U6 snRNAs. Assisted by proteins that stabilize low-fidelity, high-efficiency spliceosome conformations, the competing HEH1 5'SS are marked for alternative splicing by U5 and U6 snRNAs during the B to Bact transition of the spliceosome.
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