Evidence map›Paper›PMID 41398398›Full record

ArticleCommunications engineering2025

Reinforced optical cage systems enable drift-free single-molecule localization microscopy.

Hao Qiu, Matthew C Tang, Selene K Roberts, Guoli Li, Rong Su, Marisa L Martin-Fernandez, David T Clarke, Shugang Liu, Xiaojie Liu, Lin Wang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Communications engineering, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Hao Qiu *Central Laser Facility, Research Complex at Harwell, Rutherford Appleton Laboratory, Science and Technology Facilities Council, Didcot, UK.
Matthew C Tang *Central Laser Facility, Research Complex at Harwell, Rutherford Appleton Laboratory, Science and Technology Facilities Council, Didcot, UK.
Selene K RobertsCentral Laser Facility, Research Complex at Harwell, Rutherford Appleton Laboratory, Science and Technology Facilities Council, Didcot, UK.ORCID http://orcid.org/0000-0002-3732-0556
Guoli LiShanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai, China.
Rong SuShanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai, China.
Marisa L Martin-FernandezCentral Laser Facility, Research Complex at Harwell, Rutherford Appleton Laboratory, Science and Technology Facilities Council, Didcot, UK.
David T ClarkeCentral Laser Facility, Research Complex at Harwell, Rutherford Appleton Laboratory, Science and Technology Facilities Council, Didcot, UK.
Shugang LiuRayCage (Zhenjiang) Photoelectric Technology Co., Ltd, Zhenjiang, Jiangsu, China.
Xiaojie LiuSchool of Electrical and Information Engineering, Jiangsu University of Technology, Changzhou, Jiangsu, China.
Lin WangCentral Laser Facility, Research Complex at Harwell, Rutherford Appleton Laboratory, Science and Technology Facilities Council, Didcot, UK. lin.wang@stfc.ac.uk.ORCID http://orcid.org/0000-0001-9141-5217

Funding

RCUK | Biotechnology and Biological Sciences Research Council (BBSRC) BB/T008784/1RCUK | STFC | Central Laser Facility, Science and Technology Facilities Council (Central Laser Facility at the STFC Rutherford Appleton Laboratory) POCF2021-14RCUK | STFC | Central Laser Facility, Science and Technology Facilities Council (Central Laser Facility at the STFC Rutherford Appleton Laboratory) ST/S005404/1Rosetrees Trust Seedcorn2022\100230
6 · The paper itself

Abstract

Single-molecule localization microscopy achieves nanometer-scale resolution but is compromised by sample drift during image acquisition. Here we present reinforced optical cage systems, a novel approach that eliminates drift at its mechanical source rather than correcting it through complex image post-processing or fiducial markers. Reinforced optical cage systems employ perforated optomechanical components interconnected by tungsten-steel rods in a design proven by mechanical stability simulations. Our bench-top microscope, built with reinforced optical cage systems, demonstrated exceptional three-dimensional stability, with mean cumulative lateral drift of approximately 5 nanometers over 2 h in widefield fluorescence microscopy and 11-16 nanometers over 15 min in single-molecule localization microscopy, free from measurable axial drift. This development allows super-resolution microscopy to reach its full resolution without the necessity of sample drift correction, offering a straightforward, cost-effective, low-maintenance, and readily accessible solution to high-performance super-resolution microscopy. By addressing the fundamental issue of mechanical instability, reinforced optical cage systems enable improved precision instrumentation for the broader scientific and engineering community.

Identifiers

PMID41398398
PMCPMC12820167

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.