Evidence map›Paper›PMID 41398291›Full record

ArticleCell communication and signaling : CCS2025

BEX2 regulates autophagy by inhibiting PIK3CA-p85 interaction in non-small-cell lung cancer cells.

Yu Wang, Ning Mu, Xinke Wang, Jianhao Liu, Yiqing Qu, Xiangguo Liu, Ling Su

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In one paragraph

Article in Cell communication and signaling : CCS, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Yu WangDepartment of Pulmonary and Critical Care Medicine, Qilu Hospital of Shandong University, Jinan, 250012, China.
Ning MuShandong Provincial Key Laboratory of Animal Cell and Developmental Biology, School of Life Sciences, Shandong University, Qingdao, China.
Xinke WangShandong Provincial Hospital, Shandong First Medical University, Jinan, China.
Jianhao LiuSchool of Basic and Clinical Medicine, Shandong First Medical University, Jinan, China.
Yiqing QuDepartment of Pulmonary and Critical Care Medicine, Qilu Hospital of Shandong University, Jinan, 250012, China.
Xiangguo LiuShandong Provincial Key Laboratory of Animal Cell and Developmental Biology, School of Life Sciences, Shandong University, Qingdao, China.
Ling SuShandong Provincial Key Laboratory of Animal Cell and Developmental Biology, School of Life Sciences, Shandong University, Qingdao, China. suling@sdu.edu.cn.

Funding

National Natural Science Foundation of China 82172970National Natural Science Foundation of China 82403972Natural Science Foundation of Shandong Province ZR2023MC127Natural Science Foundation of Shandong Province ZR2023QH091
6 · The paper itself

Abstract

backgroundAutophagy is an evolutionarily conserved cellular process that maintains homeostasis. It enables tumor cells to survive and grow, which may lead to drug resistance. The PI3K/AKT/mTOR pathway is reported to play a key role in autophagy induction. BEX2 promotes cell growth and survival in cancer cells. However, its role in autophagy remains unclear.

methodsHuman non-small cell lung cancer (NSCLC) cell lines (A549, H1792 and H1299) and 293FT cells were used to investigate the function of BEX2 in autophagy. The experimental research was conducted via western blot, co-immunoprecipitation and confocal microscopy.

resultsOur data show that rapamycin induces BEX2 protein levels. Western blot and confocal microscopy analysis demonstrate that BEX2 regulates autophagy. Furthermore, BEX2 impairs PI3K/AKT/mTOR signaling. Specifically, BEX2 interacts with PIK3CA (PI3K catalytic subunit). BEX2 impairs the interaction of PIK3CA and p85 (PI3K regulatory subunit), therefore inhibiting PI3K activity. In summary, BEX2 regulates autophagy through the PI3K/AKT/mTOR signaling pathway by modulating the activity of PI3K, specifically dependent on the presence of PIK3CA.

conclusionsWe discovered that BEX2 promotes autophagic flux via PI3K/AKT/mTOR signaling. BEX2 interacts with PIK3CA and impairs PIK3CA and p85 interaction, which hinders activation of PI3K/AKT/mTOR signaling and promotes autophagy induction.

Indexed as

AutophagyCarcinoma, Non-Small-Cell LungClass Ia Phosphatidylinositol 3-KinaseClass I Phosphatidylinositol 3-KinasesLung NeoplasmsCell Line, TumorHumansProtein BindingProto-Oncogene Proteins c-aktSignal TransductionTOR Serine-Threonine KinasesClass Ia Phosphatidylinositol 3-KinaseClass I Phosphatidylinositol 3-KinasesPIK3CA protein, humanProto-Oncogene Proteins c-aktTOR Serine-Threonine KinasesAutophagyBEX2NSCLCPIK3CA

Identifiers

PMID41398291
PMCPMC12706926

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.