Evidence map›Paper›PMID 41398126›Full record

ArticleFunctional & integrative genomics2025

The application of RPA-PfAgo technology combined with multidimensional data analysis in the rapid detection of the MTHFR A1298C polymorphism.

Yaqun Liu, Lianghui Chen, Xuanyi Zheng, Peikui Yang, Jinkun Han, Jiayi Huang, Yanling Shen, Xiaotong Cai, Miaofen Fang, Yicun Chen and 3 more

Abstract read
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Article in Functional & integrative genomics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

13 authors.

Yaqun LiuGuangdong Key Laboratory of Functional Substances in Medicinal Edible Resources and Healthcare Products, Hanshan Normal University, Chaozhou, 521041, China. liuyaqun19900921@126.com.
Lianghui ChenIndustrial College of Biomedicine and Health Industry, Youjiang Medical University for Nationalities, Baise, 533000, China.
Xuanyi ZhengSecond Clinical Medical College, Southern Medical University, Guangzhou, 510000, China.
Peikui YangGuangdong Key Laboratory of Functional Substances in Medicinal Edible Resources and Healthcare Products, Hanshan Normal University, Chaozhou, 521041, China.
Jinkun HanGuangdong Key Laboratory of Functional Substances in Medicinal Edible Resources and Healthcare Products, Hanshan Normal University, Chaozhou, 521041, China.
Jiayi HuangGuangdong Key Laboratory of Functional Substances in Medicinal Edible Resources and Healthcare Products, Hanshan Normal University, Chaozhou, 521041, China.
Yanling ShenGuangdong Key Laboratory of Functional Substances in Medicinal Edible Resources and Healthcare Products, Hanshan Normal University, Chaozhou, 521041, China.
Xiaotong CaiGuangdong Key Laboratory of Functional Substances in Medicinal Edible Resources and Healthcare Products, Hanshan Normal University, Chaozhou, 521041, China.
Miaofen FangGuangdong Key Laboratory of Functional Substances in Medicinal Edible Resources and Healthcare Products, Hanshan Normal University, Chaozhou, 521041, China.
Yicun ChenShantou University Medical College, Shantou, 515000, China.
Chengsong XieGuangdong Taiantang Pharmaceutical Co., Ltd, Shantou, 515000, China.
Min LinGuangdong Key Laboratory of Functional Substances in Medicinal Edible Resources and Healthcare Products, Hanshan Normal University, Chaozhou, 521041, China.
Yuzhong ZhengGuangdong Key Laboratory of Functional Substances in Medicinal Edible Resources and Healthcare Products, Hanshan Normal University, Chaozhou, 521041, China. zhengyuzhong@gmail.com.

Funding

Chaozhou Science and Technology Bureau Science and Technology Project 202404GY06Guangdong Key Laboratory of Functional Substances in Medicinal Edible Resources and Healthcare Products 2021B1212040015Guangdong Provincial Department of Education Project 2025KQNCX043Hanshan Normal University Quality Engineering Program HSJG-SZ252187Hanshan Normal University Scientific Research Mentor Support Program XBF202302
6 · The paper itself

Abstract

This study presents a novel approach that integrates recombinase polymerase amplification (RPA) with PfAgo protein technology for the rapid and precise detection of the MTHFR A1298C polymorphism. Although traditional genotyping methods are effective, they are often limited by complexity, high cost, and the need for specialized equipment. The RPA-PfAgo technique harnesses the swift isothermal amplification of RPA and the high specificity and sensitivity of PfAgo-mediated DNA cleavage, completing the entire process from sample collection to detection within 90 min. The utility of this method has been substantiated through a battery of optimization experiments, parameter analysis, and assessments of sensitivity, specificity, and repeatability, along with clinical validation using oral mucosal samples. These findings indicate that this new technology not only substantially reduces detection time and cost but also offers an effective tool for personalized medicine and disease prevention with high accuracy and reliability.

Indexed as

Methylenetetrahydrofolate Reductase (NADPH2)Nucleic Acid Amplification TechniquesPolymorphism, Single NucleotideRecombinasesHumansMethylenetetrahydrofolate Reductase (NADPH2)MTHFR protein, humanRecombinasesGenotypingMTHFR A1298C polymorphismMultidimensional data analysisPfAgo proteinRecombinase polymerase amplification (RPA)

Identifiers

PMID41398126

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.