Evidence map›Paper›PMID 41398086›Full record

ArticleParasitology research2025

Prevalence of porcine toxoplasmosis using ERP-iELISA in Hubei Province.

Linyue Wu, Mingfeng He, Yu Wang, Xiaodie Ruan, Liyan Luo, Bang Shen, Rui Fang, Junlong Zhao, Yanqin Zhou

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Article in Parasitology research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Linyue Wu *Key Laboratory Preventive Veterinary of Hubei Province, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, People's Republic of China.
Mingfeng He *State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, 430070, People's Republic of China.
Yu WangKey Laboratory Preventive Veterinary of Hubei Province, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, People's Republic of China.
Xiaodie RuanKey Laboratory Preventive Veterinary of Hubei Province, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, People's Republic of China.
Liyan LuoState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, 430070, People's Republic of China.
Bang ShenState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, 430070, People's Republic of China.
Rui FangState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, 430070, People's Republic of China.
Junlong ZhaoKey Laboratory Preventive Veterinary of Hubei Province, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, People's Republic of China.
Yanqin ZhouKey Laboratory Preventive Veterinary of Hubei Province, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, People's Republic of China. yanqinzhou@mail.hzau.edu.cn.

Funding

National Key Research and Development Program of China 2022YFE0114400
6 · The paper itself

Abstract

Toxoplasma gondii (T. gondii) is an intracellular parasite that can infect almost all mammalian species, including humans and important economic animals such as pigs and sheep, thereby causing serious public health security issues. In pigs, such infections are common and economically significant, yet distinguishing between infection routes remains challenging. The current commonly used detection methods mainly aim to quickly detect positive cases of toxoplasmosis but cannot distinguish between different modes of infection. To distinguish whether cysts or oocysts are the source of T. gondii infection, the ERP protein that specifically expressed in T. gondii sporulated oocysts stagewas applied to develop an indirect enzyme-linked immunosorbent assay (iELISA) for specific detection of oocyst-derived porcine toxoplasmosis. This study used the TgGRA1-iELISA and TgERP-iELISA detection methods to conduct a serological survey of toxoplasmosis in pigs in 17 municipalities of Hubei Province, with a total of 2,303 sera samples from 115 farms. Serological detection results demonstrated that TgGRA1-iELISA detected 744 positive samples, with a detection rate of 32.31%, and TgERP-iELISA detected 383 positive samples, with a detection rate of 16.63%, and all positive samples were positive for GRA1 antibody. This indicates that the modes of Toxoplasmosis infection in pigs in Hubei Province are through both cyst infection and oocyst infection. Furthermore, the detection rate of toxoplasmosis varies significantly across different regions. This study provides the first large-scale evidence of oocyst-derived porcine toxoplasmosis in Hubei Province and establishes a tool to support source-targeted prevention strategies in pig farms.

Indexed as

Antigens, ProtozoanProtozoan ProteinsSwine DiseasesToxoplasmaToxoplasmosis, AnimalAnimalsAntibodies, ProtozoanChinaEnzyme-Linked Immunosorbent AssayOocystsPrevalenceSeroepidemiologic StudiesSwineAntibodies, ProtozoanAntigens, ProtozoanProtozoan ProteinsERPGRA1IELISAOocystToxoplasma gondii

Identifiers

PMID41398086
PMCPMC12705833

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.