Evidence map›Paper›PMID 41397992›Full record

ArticleNature communications2025

LncRNA CISTR-ACT regulates cell size in human and mouse by guiding FOSL2.

Katerina Kiriakopulos, Katty Soleimanpour, Brandon J McMurray, Benjamin-Israel Moke, Jordan J Chalmers, Milad Mokhtaridoost, Jeremy D Newton, Taylor De Young, Kate Delfosse, Mai Ahmed and 8 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Katerina KiriakopulosGenetics and Genome Biology Program, The Hospital for Sick Children, Toronto, ON, Canada.ORCID http://orcid.org/0009-0005-2345-750X
Katty SoleimanpourGenetics and Genome Biology Program, The Hospital for Sick Children, Toronto, ON, Canada.ORCID http://orcid.org/0009-0008-5524-6928
Brandon J McMurrayGenetics and Genome Biology Program, The Hospital for Sick Children, Toronto, ON, Canada.ORCID http://orcid.org/0000-0003-3394-0667
Benjamin-Israel MokeDepartment of Molecular Genetics, University of Toronto, Toronto, ON, Canada.
Jordan J ChalmersGenetics and Genome Biology Program, The Hospital for Sick Children, Toronto, ON, Canada.ORCID http://orcid.org/0000-0002-8656-3386
Milad MokhtaridoostGenetics and Genome Biology Program, The Hospital for Sick Children, Toronto, ON, Canada.ORCID http://orcid.org/0000-0001-6185-4251
Jeremy D NewtonDepartment of Mechanical Engineering, University of Toronto, Toronto, ON, Canada.ORCID http://orcid.org/0000-0002-3691-5152
Taylor De YoungMouse Imaging Center, The Hospital for Sick Children, Toronto, ON, Canada.
Kate DelfosseGenetics and Genome Biology Program, The Hospital for Sick Children, Toronto, ON, Canada.
Mai AhmedProgram in Developmental, Stem Cell, and Cancer Biology, The Hospital for Sick Children, Toronto, ON, Canada.
Cassandra J WongLunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Sinai Health System, Toronto, ON, Canada.ORCID http://orcid.org/0009-0009-7292-3780
Sigmar StrickerInstitute for Chemistry and Biochemistry, Freie Universität Berlin, Berlin, Germany.ORCID http://orcid.org/0000-0002-7174-5363
Yun LiDepartment of Molecular Genetics, University of Toronto, Toronto, ON, Canada.ORCID http://orcid.org/0000-0002-5849-5910
Brian J NiemanMouse Imaging Center, The Hospital for Sick Children, Toronto, ON, Canada.
Anne-Claude GingrasDepartment of Molecular Genetics, University of Toronto, Toronto, ON, Canada.ORCID http://orcid.org/0000-0002-6090-4437
Monica J JusticeGenetics and Genome Biology Program, The Hospital for Sick Children, Toronto, ON, Canada.ORCID http://orcid.org/0000-0003-3562-2099
Julie L LefebvreDepartment of Molecular Genetics, University of Toronto, Toronto, ON, Canada.ORCID http://orcid.org/0000-0002-3110-860X
Philipp G MaassGenetics and Genome Biology Program, The Hospital for Sick Children, Toronto, ON, Canada. philipp.maass@sickkids.ca.ORCID http://orcid.org/0000-0002-2742-8301

Funding

Gouvernement du Canada | Canadian Institutes of Health Research (Instituts de Recherche en Santé du Canada) CIHR PJT 173542
6 · The paper itself

Abstract

Organisms regulate cell size and shape to function efficiently. Aberrant cell morphogenesis is commonly associated with disease, yet gene-regulatory mechanisms remain unknown. CISTR-ACT was the first lncRNA involved in inter-chromosomal proximities and Mendelian disease, and it is associated with mean corpuscular volume (red blood cell size). Here, functional dissection of CISTR-ACT's DNA- and RNA-encoded mechanisms by in vitro and in vivo perturbations reveals that CISTR-ACT regulates cell size across cell types and species. CISTR-ACT's locus is embedded in a stable inter-chromosomal environment which contains cell size genes that are regulated by CISTR-ACT in trans. CISTR-ACT's RNA also has function and directly interacts with transcription factor FOSL2 to guide its regulation of cell morphogenesis and cell-cell adhesion genes. In the absence of CISTR-ACT, the FOSL2-chromatin binding is perturbed. Our study exemplifies how a functionally conserved lncRNA regulates cell size with multiple modes of action and ultimately contributes to clinically relevant phenotypes.

Indexed as

Cell SizeFos-Related Antigen-2RNA, Long NoncodingAnimalsCell AdhesionChromatinGene Expression RegulationHumansMiceChromatinFOSL2 protein, humanFosl2 protein, mouseFos-Related Antigen-2RNA, Long Noncoding

Identifiers

PMID41397992
PMCPMC12827430

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.