Evidence map›Paper›PMID 41397308›Full record

ArticleUltrasonics sonochemistry2026

Exploring the synergistic potential of pH and ultrasonication on the functional properties of pea and lentil protein isolates and its formulation in food product.

Mehwish Arshad, Rana Muhammad Aadil, Moazzam Rafiq Khan, Abid Aslam Maan, Anubhav Pratap-Singh

Abstract read
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Article in Ultrasonics sonochemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Mehwish ArshadNational Institute of Food Science and Technology, University of Agriculture, Faisalabad, Pakistan.
Rana Muhammad AadilNational Institute of Food Science and Technology, University of Agriculture, Faisalabad, Pakistan. Electronic address: muhammad.aadil@uaf.edu.pk.
Moazzam Rafiq KhanNational Institute of Food Science and Technology, University of Agriculture, Faisalabad, Pakistan.
Abid Aslam MaanNational Institute of Food Science and Technology, University of Agriculture, Faisalabad, Pakistan.
Anubhav Pratap-SinghFood, Nutrition, and Health, Faculty of Land and Food Systems, The University of British Columbia, 2205 East Mall, Vancouver V6T 1Z4 BC Canada. Electronic address: anubhav.singh@ubc.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The substitution of meat proteins with plant-based proteins from various sources is often motivated by nutritional considerations. However, the inherent limited solubility of plant proteins, which results in suboptimal techno-functional properties, remains a persistent challenge in food formulation. The purpose of this study was to utilize unique properties of pea (Pisum sativum L.) and lentil (Lens culinaris) through ultrasonication and pH variation in order to develop a stable and consumable protein-based product. For this purpose, pea and lentils powders were subjected to salt extraction (SE) and proceeded to further treatment with ultrasonication and pH shifting at pH 2, 4, 10 and 12 with ultrasound assisted extraction (UAE) at 20 kHz at 20 °C for 5 min for each treatment. The developed protein isolates (PIs) after these treatments were analyzed for functional properties including surface hydrophobicity, solubility, foaming capacity and stability, emulsifying activity and stability indices, turbidity and molecular weight. Among these treatments, the pH-12 treated samples showed optimal values for the functional profile, and this treatment was selected for further development of meat-analogous nuggets through assorted combinations of pea protein isolate (PPI) and lentil protein isolate (LPI) along with control treatment containing chicken nuggets. After development, nuggets were further tested for moisture content, total plate count and textural hardness for a storage period of 4 weeks along with sensory profile analyses of the developed nuggets. Meat-analogous nuggets prepared from 60 % LPI and 40 % PPI had an optimal response to texture profile and were also favored in sensory profile. This product might help the protein requirement as a vegan-based diet and enhance peas and lentils productivity into a marketable and nourishing product if it were to be developed more widely and commercialized.

Indexed as

Food HandlingLens PlantPea ProteinsPisum sativumPlant ProteinsSonicationHydrogen-Ion ConcentrationHydrophobic and Hydrophilic InteractionsSolubilityPea ProteinsPlant ProteinsMeat analoguesNon-thermal techniquesProtein isolatesVegan alternatives ultrasonicationVegan nuggets

Identifiers

PMID41397308
PMCPMC12767791

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LicenceCC BY-NC-ND
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.