ArticleJournal of visualized experiments : JoVE2025
Spatial Molecular Imaging of the Glycome Using Mass Spectrometry.
Article in Journal of visualized experiments : JoVE, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- Heparan Sulfate Proteoglycans: Master Regulators of Cellular Signaling, Tissue Development, and Neural Function.Journal of neuroscience research · 2026Review
- Mass Spectrometry-Based Spatial Imaging of the Cochlea.Journal of the American Society for Mass Spectrometry · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors.
Funding
Abstract
The spatial organization of the glycome within tissues is key to the molecular basis for physiological function. The diverse and dynamic glycome is critical for fundamental cellular processes, including metabolism, signaling, and adhesion. Innovations in spatial biology have ushered in new avenues for spatial molecular imaging of diverse glycome classes. Here, we describe an optimized protocol for spatial biomolecular imaging of the glycome in fresh-frozen mouse liver. The workflow comprises (1) rapid harvesting and freezing, (2) cryostat sectioning at optimal thickness and position, (3) tissue preparation and on-tissue enzyme digestion using carbohydrate-active enzymes, (4) matrix application and data acquisition by matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI), and (5) data processing and visualization to place the findings in biological context. Use of this approach allows acquisition of detailed spatial maps of N-linked glycans and glycogen, revealing key physiological and cellular features. These data allow the definition of key spatial glycomic heterogeneity associated with liver function and dysfunction. This workflow enables highly reproducible and sensitive spatial glycomics of the mouse liver. Additionally, it is readily adaptable to other tissues or species, facilitating novel spatial insights into glycome biology in health and disease.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.