ArticleSmall science2025
Hydrostatic Pressure Induces Osteogenic Differentiation of Single Stem Cells in 3D Viscoelastic Microgels.
Article in Small science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
1 citing paper in PubMed.
- Hydrostatic Pressure Induces Osteogenic Differentiation of Single Stem Cells in 3D Viscoelastic Microgels.Small science · 2025Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sustained mechanical stimulation represents a powerful strategy for directing stem cell fate, yet its application within microscale injectable carriers remains limited. This study presents a dynamic microgel platform enabling osteogenic differentiation of single mesenchymal stem cells (MSCs) solely through hydrostatic pressure, without biochemical induction. Individual MSCs are encapsulated in ionically crosslinked, cell-adhesive alginate microgels and stabilized using an alginate-poly-l-lysine-alginate and calcium coating. Application of cyclic hydrostatic pressure at 200 kPa and 0.5 Hz frequency for 30 min per day leads to upregulation of early osteogenic markers RUNX2 and alkaline phosphatase, enhanced collagen I synthesis, and mineralization over 21 days. Results demonstrate that mechanical cues alone are sufficient to orchestrate osteogenic commitment in soft, confined microenvironments, offering a scalable approach to stem cell programming. This work establishes a versatile, high-resolution platform for engineering lineage specification at the single-cell level and highlights the potential of force-driven strategies for scalable production of therapeutic stem cells.
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Registered trials
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