ArticleClinical & translational immunology2025
An optimised whole blood interleukin-2 release assay is more sensitive than interferon-γ ELISpot for detecting and quantifying gluten-specific CD4
Article in Clinical & translational immunology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Objectives: Detection of antigen-specific T cells has been crucial for the study of autoimmune illnesses such as coeliac disease (CD). In CD, an oral gluten challenge is required to expand the rare population of circulating gluten-specific T cells to detectable levels for IFN-γ ELISpot detection. We evaluated interleukin (IL)-2 detection as a simpler approach to identify gluten-specific T cells in CD. Methods: HLA-DQ2.5+-treated CD adults ( Results: GCIL-2 was 90% sensitive in detecting CD. Day 6 post challenge was the optimal day for assessment. Conclusions: The IL-2 WBA provides superior sensitivity over IFN-γ ELISpot for detecting gluten-specific T cells. It can measure the functional blockade of HLA and accurately reflects gluten peptide immunogenicity hierarchies. The IL-2 WBA supports immunomonitoring and T-cell epitope mapping in CD and offers broad research and clinical application beyond CD.
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