Evidence map›Paper›PMID 41394403›Full record

ArticleFrontiers in cell and developmental biology2025

Tissue block-resolved developmental transcriptomic atlas of human fetal brainstem reveals gene modules with implications for neurological disorders.

Chengxin Liu, Wenjuan Zhou, Xin Xing, Jiachen Chen, Chenxi Sun, Shizhou Liu, Yunxia Lou, Jianfen Jiao, Haoling Cao, Baoxia Cui and 3 more

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In one paragraph

Article in Frontiers in cell and developmental biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

13 authors.

Chengxin LiuDepartment of Anatomy and Neurobiology, Key Laboratory of Experimental Teratology of the Ministry of Education, Shandong Key Laboratory of Mental Disorders and Intelligent Control, Shandong Key Laboratory of Digital Human and Clinical Anatomy, School of Basic Medical Sciences, Cheeloo College of Medicine, Institute for Sectional Anatomy and Digital Human, Shandong University, Jinan, Shandong, China.
Wenjuan ZhouDepartment of Anatomy and Neurobiology, Key Laboratory of Experimental Teratology of the Ministry of Education, Shandong Key Laboratory of Mental Disorders and Intelligent Control, Shandong Key Laboratory of Digital Human and Clinical Anatomy, School of Basic Medical Sciences, Cheeloo College of Medicine, Institute for Sectional Anatomy and Digital Human, Shandong University, Jinan, Shandong, China.
Xin XingDepartment of Obstetrics and Gynecology, The First Affiliated Hospital of Shandong First Medical University and Shandong Provincial Qianfoshan Hospital, Jinan, Shandong, China.
Jiachen ChenDepartment of Anatomy and Neurobiology, Key Laboratory of Experimental Teratology of the Ministry of Education, Shandong Key Laboratory of Mental Disorders and Intelligent Control, Shandong Key Laboratory of Digital Human and Clinical Anatomy, School of Basic Medical Sciences, Cheeloo College of Medicine, Institute for Sectional Anatomy and Digital Human, Shandong University, Jinan, Shandong, China.
Chenxi SunDepartment of Anatomy and Neurobiology, Key Laboratory of Experimental Teratology of the Ministry of Education, Shandong Key Laboratory of Mental Disorders and Intelligent Control, Shandong Key Laboratory of Digital Human and Clinical Anatomy, School of Basic Medical Sciences, Cheeloo College of Medicine, Institute for Sectional Anatomy and Digital Human, Shandong University, Jinan, Shandong, China.
Shizhou LiuDepartment of Anatomy and Neurobiology, Key Laboratory of Experimental Teratology of the Ministry of Education, Shandong Key Laboratory of Mental Disorders and Intelligent Control, Shandong Key Laboratory of Digital Human and Clinical Anatomy, School of Basic Medical Sciences, Cheeloo College of Medicine, Institute for Sectional Anatomy and Digital Human, Shandong University, Jinan, Shandong, China.
Yunxia LouDepartment of Ultrasound, Qilu Hospital of Shandong University, Jinan, Shandong, China.
Jianfen JiaoDepartment of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan, Shandong, China.
Haoling CaoDepartment of Anatomy and Neurobiology, Key Laboratory of Experimental Teratology of the Ministry of Education, Shandong Key Laboratory of Mental Disorders and Intelligent Control, Shandong Key Laboratory of Digital Human and Clinical Anatomy, School of Basic Medical Sciences, Cheeloo College of Medicine, Institute for Sectional Anatomy and Digital Human, Shandong University, Jinan, Shandong, China.
Baoxia CuiDepartment of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan, Shandong, China.
Shuhui HongDepartment of Obstetrics and Gynecology, The First Affiliated Hospital of Shandong First Medical University and Shandong Provincial Qianfoshan Hospital, Jinan, Shandong, China.
Niloufar AhmadiDepartment of Anatomy and Neurobiology, Key Laboratory of Experimental Teratology of the Ministry of Education, Shandong Key Laboratory of Mental Disorders and Intelligent Control, Shandong Key Laboratory of Digital Human and Clinical Anatomy, School of Basic Medical Sciences, Cheeloo College of Medicine, Institute for Sectional Anatomy and Digital Human, Shandong University, Jinan, Shandong, China.
Yuchun TangDepartment of Anatomy and Neurobiology, Key Laboratory of Experimental Teratology of the Ministry of Education, Shandong Key Laboratory of Mental Disorders and Intelligent Control, Shandong Key Laboratory of Digital Human and Clinical Anatomy, School of Basic Medical Sciences, Cheeloo College of Medicine, Institute for Sectional Anatomy and Digital Human, Shandong University, Jinan, Shandong, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: The brainstem is a critical hub connecting the cerebrum and spinal cord. However, the gene regulatory dynamics during fetal brainstem development remain incompletely characterized. Methods: This study employed RNA-seq to map transcriptomes across 107 tissue blocks from 18 human fetal brainstems (gestational weeks 9-33). Weighted gene co-expression network analysis (WGCNA) identified 22 functionally annotated modules. We quantitatively assessed their spatiotemporal activity gradients and systematically classified genes exhibiting significant temporal trajectories based on phase-specific signatures. Results: Our integrated approach constructed a developmental transcriptomic profile, revealing stage-specific regulatory networks and dynamic transcriptional trajectories governing ontogeny. Crucially, we validated the expression of neurodevelopmental disorder-associated genes within fetal brainstem tissues. Discussion: This work advances our understanding of brainstem development and provides a foundational resource for research into neurological disorders.

Indexed as

brainstemfetal developmentfetal gene expressiongene regulationRNA sequencing

Identifiers

PMID41394403
PMCPMC12698620

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.