ArticleFrontiers in cellular and infection microbiology2025
Development of Multiplex PCR assay for detection of Canine Infectious Respiratory Disease Complex (CIRDC) pathogens in dogs.
Article in Frontiers in cellular and infection microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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Who cites it
1 citing paper in PubMed.
- A targeted PCR approach for the detection of IOLA in canine infectious respiratory disease samples during an atypical CIRD outbreak in winter 2023.Frontiers in veterinary science · 2026Article
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Authors and funding
5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Background: Canine Infectious Respiratory Disease Complex (CIRDC) is a highly contagious, multifactorial syndrome that primarily affects dogs in crowded environments such as shelters, kennels, and breeding facilities. Three major CIRDC-associated pathogens: Canine distemper virus (CDV), Canine adenovirus type 2 (CAV-2), and Materials and Methods: In this study, a multiplex PCR (mPCR) assay was developed and optimized for the simultaneous detection of these three pathogens. The multiplex assay was designed targeting three genes H, E3 and Results: The developed assay demonstrated high analytical sensitivity, detecting 1,060 copies/μL for CDV, 11,403 copies/μL for CAV-2, and 11,016 copies/μL for Bb, with 100% specificity and no cross-reactivity with non-target organisms. The assay was validated on 55 clinical samples of dogs suspected with CIRDC, the assay detected pathogens in 32.2% of cases, with CDV being the most prevalent (25%). Compared with previously published singleplex PCR methods, the mPCR showed excellent diagnostic performance, achieving 94.12% sensitivity, 94.74% specificity, and 94.55% overall accuracy. Conclusion: This study demonstrates a rapid, specific, and cost-effective diagnostic mPCR assay capable of efficiently identifying key CIRDC pathogens in a single reaction. The assay is highly suitable for molecular diagnosis as well as large-scale field surveillance.
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