ArticleFrontiers in medicine2025
Recovery efficiency and functional characterization of T cells and NK cells from leukocyte filters.
Article in Frontiers in medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Objective: This study aimed to investigate the recovery efficiency and functional characteristics of lymphocytes isolated from leukocyte filters, compared with those isolated directly from whole blood sample, and to assess their potential as an alternative immune cell source for therapeutic applications. Methods: From December 2023 to 2024, leukocyte filters and 5 mL whole blood samples were collected from healthy donors. Cell recovery was optimized by testing different flushing methods (forward/reverse), solutions (1 × PBS/NS), and volumes (20 - 80 mL). Peripheral blood mononuclear cells (PBMCs) were isolated via density gradient centrifugation, and lymphocyte subsets (CD3 Results: Reverse flushing with 40 mL normal saline (NS) achieved cost-effective lymphocyte recovery, with cell counts correlating with flushing method and volume. The proportion of CD3 Conclusion: Reverse flushing of leukocyte filters with 40 mL NS enables cost-effective recovery of lymphocytes, particularly CD3
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