Visualizing intracellular milieu and electric field-induced cell responses by fluorescence lifetime imaging microscopy.

Kamlesh Awasthi, Wei-Chen Huang, Hsin-Yun Hsu, Nobuhiro Ohta

Abstract read

In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Kamlesh AwasthiDepartment of Applied Chemistry, Institute of Molecular Science, National Yang Ming Chiao Tung University, 1001 Ta-Hsueh Rd., Hsinchu, 300093, Taiwan. kaawasthi@gmail.com.

Wei-Chen HuangDepartment of Applied Chemistry, Institute of Molecular Science, National Yang Ming Chiao Tung University, 1001 Ta-Hsueh Rd., Hsinchu, 300093, Taiwan.

Hsin-Yun HsuDepartment of Applied Chemistry, Institute of Molecular Science, National Yang Ming Chiao Tung University, 1001 Ta-Hsueh Rd., Hsinchu, 300093, Taiwan.

Nobuhiro OhtaDepartment of Applied Chemistry, Institute of Molecular Science, National Yang Ming Chiao Tung University, 1001 Ta-Hsueh Rd., Hsinchu, 300093, Taiwan. nohta@nycu.edu.tw.

Funding

National Science and Technology Council 113-2113-M-A49-021, 114-2113-M-A49-013

6 · The paper itself

Abstract

Fluorescence-based optical techniques have significantly advanced our comprehension of living systems, providing invaluable insights into cellular intricacies and facilitating fundamental research and practical diagnostic applications. To explore intracellular environments and the communication among intracellular organelles, we utilized fluorescence lifetime imaging microscopy with exogenous fluorophores of Oregon Green 488 BAPTA-1 AM and Fluovolt and endogenous fluorophores of NAD(P)H and FAD in breast carcinoma MCF7 and MDA-MB-231 and normal MCF10A cells. Fluorescence lifetime images have uncovered a characteristic triangular interrelationship among intracellular calcium ion concentration (Ca

Indexed as

Breast NeoplasmsCalciumCell Line, TumorElectricityFemaleFlavin-Adenine DinucleotideHumansMCF-7 CellsMicroscopy, FluorescenceMitochondriaCalciumFlavin-Adenine Dinucleotide

Identifiers

PMID41392207

DOI10.1038/s41598-025-32020-y

PMCPMC12816114

What OpenQuestion holds

Textmetadata

LicenceCC BY-NC-ND

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.