ArticleFEBS letters2026
Substrate specificity of Burkholderia pseudomallei multidrug transporters is influenced by the hydrophilic patch in the substrate-binding pocket.
Article in FEBS letters, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
The Gram-negative pathogen Burkholderia pseudomallei possesses multiple resistance-nodulation-division superfamily transporters that contribute to multidrug resistance, including BpeB and BpeF. Structural studies of BpeB and BpeF have identified a hydrophilic patch in their substrate-binding pocket. To investigate the relationship between this hydrophilic patch and substrate specificity, mutant analyses were performed using an Escherichia coli recombinant expression system. Drug susceptibility tests of BpeB and BpeF mutants showed up to a 64-fold increase in susceptibility compared with the wild type. Growth curve analyses revealed that BpeB mutants exhibited increased resistance to aminoglycosides, which are not transported by the wild type. These findings suggest that the hydrophilic patches in the substrate-binding pockets of BpeB and BpeF are involved in the substrate specificity.
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