Evidence map›Paper›PMID 41390851›Full record

ArticleScientific reports2025

A human iPSC-based neural spheroid platform for modelling glioblastoma infiltration using high-content imaging.

Victoria S K Tsang, Federica Riccio, Aimee S Wilson, Hannah Nudds, Jason D Coombes, Heiko Wurdak, Harry J C J Bulstrode, Ivo Lieberam, Davide Danovi

Abstract read
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In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Victoria S K TsangCentre for Gene Therapy and Regenerative Medicine, King's College London, London, UK. v.tsang@qmul.ac.uk.
Federica RiccioCentre for Gene Therapy and Regenerative Medicine, King's College London, London, UK.
Aimee S WilsonWellcome MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK.
Hannah NuddsCentre for Gene Therapy and Regenerative Medicine, King's College London, London, UK.
Jason D CoombesCentre for Gene Therapy and Regenerative Medicine, King's College London, London, UK.
Heiko WurdakSchool of Medicine, University of Leeds, Leeds, UK.
Harry J C J BulstrodeWellcome MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK.
Ivo LieberamCentre for Gene Therapy and Regenerative Medicine, King's College London, London, UK.
Davide DanoviCentre for Gene Therapy and Regenerative Medicine, King's College London, London, UK.

Funding

Innovate UK TSB/89370Medical Research Council MR/N025865/1Wellcome Trust 108874/Z/15/ZWellcome Trust 223011
6 · The paper itself

Abstract

Glioblastoma is the most aggressive adult brain tumour, characterised by resistance to therapy and high recurrence due to diffuse infiltration. We developed a physiologically relevant co-culture model, combining patient-derived glioblastoma cell lines with cortical-like neural spheroids differentiated from human induced pluripotent stem cells. Using high-content imaging, we demonstrate that GBM20 and GBM1 cell lines migrate directionally along axons toward neural spheroids in live imaging assays and infiltrate spheroids extensively in endpoint assays, unlike non-cancerous neural stem cells. A proof-of-principle drug screen identified PF 573228 (FAK inhibitor) and motixafortide (CXCR4 inhibitor) as potent suppressors of GBM20 and GBM1 infiltration, respectively. Bulk RNA sequencing revealed gene expression profiles correlating with invasive behaviour and drug sensitivity. This platform offers a valuable model for studying glioblastoma infiltration along axons and provides proof-of-principle that migration can serve as a measurable and actionable phenotype to screen therapeutic vulnerabilities in glioblastoma.

Indexed as

Brain NeoplasmsGlioblastomaInduced Pluripotent Stem CellsSpheroids, CellularCell DifferentiationCell Line, TumorCell MovementCoculture TechniquesHumansNeural Stem CellsReceptors, CXCR4Receptors, CXCR4Cancer migrationGlioblastomaHigh-content imagingInduced-pluripotent stem cellsNeural spheroidStem cell modelling

Identifiers

PMID41390851
PMCPMC12789424

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.