Evidence map›Paper›PMID 41390424›Full record

ArticleStem cell research & therapy2025

Deciphering the gonadal cell atlas of Monopterus albus and cell fate during sex reversal based on single-cell RNA sequencing.

Lingbo Yi, Fuyun Zhu, Lun Liu, Runlong Zhao, Yanhong Luo, Shuqing Liang, Qingtao Yang, Jihua Ye, Haibo Yang, Li Wang and 2 more

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In one paragraph

Article in Stem cell research & therapy, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

12 authors.

Lingbo Yi *Department of Hematology, School of Basic Medical Sciences, The First Affiliated Hospital of Chongqing Medical University, Chongqing Medical University, Chongqing, 400016, China.
Fuyun Zhu *Department of Hematology, School of Basic Medical Sciences, The First Affiliated Hospital of Chongqing Medical University, Chongqing Medical University, Chongqing, 400016, China.
Lun LiuDepartment of Hematology, School of Basic Medical Sciences, The First Affiliated Hospital of Chongqing Medical University, Chongqing Medical University, Chongqing, 400016, China.
Runlong ZhaoDepartment of Hematology, School of Basic Medical Sciences, The First Affiliated Hospital of Chongqing Medical University, Chongqing Medical University, Chongqing, 400016, China.
Yanhong LuoAffiliated Hospital of Zunyi Medical University, Zunyi, 563000, Guizhou, China.
Shuqing LiangHealth Time Gene Institute, Shenzhen, 518000, Guangdong, China.
Qingtao YangInformation Center, Chongqing Medical University, Chongqing, 400016, China.
Jihua YeChongqing Qibang Technology Co., Ltd, Chongqing, 400016, China.
Haibo YangInformation Center, Chongqing Medical University, Chongqing, 400016, China.
Li WangDepartment of Hematology, School of Basic Medical Sciences, The First Affiliated Hospital of Chongqing Medical University, Chongqing Medical University, Chongqing, 400016, China. liwang@hospital.cqmu.edu.cn.
Yu HouDepartment of Hematology, School of Basic Medical Sciences, The First Affiliated Hospital of Chongqing Medical University, Chongqing Medical University, Chongqing, 400016, China. houyu@cqmu.edu.cn.
Xueya ZhaoDepartment of Hematology, School of Basic Medical Sciences, The First Affiliated Hospital of Chongqing Medical University, Chongqing Medical University, Chongqing, 400016, China. 103137@cqmu.edu.cn.ORCID http://orcid.org/0009-0000-5547-261X

Funding

Chongqing Municipal Education Commission Grant No.KJQN202300444National Natural Science Foundation of China 31900588Natural Science Foundation of Chongqing Municipality CSTB2022NSCQ-MSX1037
6 · The paper itself

Abstract

backgroundThe Monopterus albus serves as a unique model for studying sex reversal, transitioning naturally from female to male. However, the origins of male germline stem cells (GSCs) and the roles of somatic cells during sex reversal remain poorly understood.

methodsWe performed single-cell RNA sequencing (scRNA-seq) on ovarian, ovotestis, and testicular tissues to construct a gonadal cell atlas. RNA fluorescence in situ hybridization (RNA-FISH) validated cell subpopulations.

resultsCell types of germ cells and somatic cells in gonads were identified, and their differentiation trajectories during sex reversal were depicted. Our results show that GSCs

conclusionsThis study provides a comprehensive cellular roadmap of gonadal cell dynamics during sex reversal in Monopterus albus. Our findings unveil the molecular mechanisms underlying germline and somatic cell fate determination, offering novel insights into vertebrate sex reversal and potential therapeutic strategies for disorders of sex development (DSD).

Indexed as

GonadsSex Determination ProcessesSingle-Cell AnalysisSmegmamorphaAnimalsCell DifferentiationFemaleMaleOvarySequence Analysis, RNASex DifferentiationTestisCell plasticityGSCsMonopterus albusscRNA-seqSex reversal

Identifiers

PMID41390424
PMCPMC12821253

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