Evidence map›Paper›PMID 41390370›Full record

ArticleBMC genomic data2025

Complete genome sequence and genetic features of a novel Pseudomonas sp. isolate (CAM1A) from tsetse fly gut captured in Dodeo, Cameroon.

Youssouf Mouliom Mfopit, Judith Sophie Engel, Rolf Nimzyk, Andrea Schaffrath, Gloria Dada Chechet, Petra Berger, Mahamat Alhadj Moussa Ibrahim, Daniel Mbunkah Achukwi, Mohammed Mamman, Emmanuel Oluwadare Balogun and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomic data, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Draft genome sequence ofMicrobiology resource announcements · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Youssouf Mouliom MfopitInstitute of Agricultural Research for Development (IRAD), Yaounde, Cameroon. youssoufmfopit@yahoo.fr.
Judith Sophie EngelMARUM-PANGAEA, University of Bremen, Bremen, Germany.
Rolf NimzykCentre for Biomolecular Interactions Bremen, Faculty of Biology and Chemistry, University of Bremen, Bremen, Germany.
Andrea SchaffrathCentre for Biomolecular Interactions Bremen, Faculty of Biology and Chemistry, University of Bremen, Bremen, Germany.
Gloria Dada ChechetDepartment of Biochemistry, Ahmadu Bello University, Zaria, Nigeria.
Petra BergerCentre for Biomolecular Interactions Bremen, Faculty of Biology and Chemistry, University of Bremen, Bremen, Germany.
Mahamat Alhadj Moussa IbrahimDepartment of Public Health, University of N'Djamena, N'Djamena, Chad.
Daniel Mbunkah AchukwiImmuno-Parasitology Section, TOZARD Research Laboratory, Bamenda, Cameroon.
Mohammed MammanAfrica Centre of Excellence for Neglected Tropical Diseases and Forensic Biotechnology (ACENTDFB), Zaria, Nigeria.
Emmanuel Oluwadare BalogunDepartment of Biochemistry, Ahmadu Bello University, Zaria, Nigeria.
Mohammed Nasir ShuaibuDepartment of Biochemistry, Ahmadu Bello University, Zaria, Nigeria.
Junaidu KabirAfrica Centre of Excellence for Neglected Tropical Diseases and Forensic Biotechnology (ACENTDFB), Zaria, Nigeria.
Barbara Reinhold-HurekCentre for Biomolecular Interactions Bremen, Faculty of Biology and Chemistry, University of Bremen, Bremen, Germany.
Sørge KelmCentre for Biomolecular Interactions Bremen, Faculty of Biology and Chemistry, University of Bremen, Bremen, Germany.

Funding

Multi-target approach to rational design of novel therapeutics for human African trypanosomiasisK43TW012015 · FIC · AHMADU BELLO UNIVERSITY · PI BALOGUN, EMMANUEL OLUWADARE · 2021 to 2025
$577k
Agence Française de Développement WAN100629XDeutsche Forschungsgemeinschaft Ke428/13-1Deutsche Forschungsgemeinschaft Ke428/8-1FIC NIH HHS K43 TW012015NIH National Institutes of Health & Fogarty International Center K43-TW012015
6 · The paper itself

Abstract

Bacteria of the genus Pseudomonas are Gram-negative and comprise of at least 107 diverse species. Certain members of the genus Pseudomonas emerged as major opportunistic human pathogens, and others have been used as biocontrol agents. Tsetse flies, the biological vectors of African trypanosomes, harbour diverse communities of microorganisms. Tsetse microbiota is of interest because of their possible effect on vector competence. The aim of this study was to explore genetic features of a novel Pseudomonas species strain CAM1A isolated from tsetse caught in Dodeo, Cameroon. The bacterium was isolated from tsetse fly, and the whole genome was sequenced on the Illumina Miseq platform. Sequences were assembled with SPAdes and annotated using RAST and PATRIC annotation pipelines. The draft genome, made of 61 contigs was found to be at least 5,848,000 bp long, with 5,339 coding sequences, a DNA G + C content of 64.3%, and at least 80 tRNA + rRNA genes. No plasmid was found. Taxonomic analysis based on whole-genome sequences (ANI and GGDC) and MLSA indicated that CAM1A represents a putative novel species closely related to Pseudomonas soli. Several antimicrobial resistance and efflux-mediated resistance genes were predicted, suggesting its ability to evade many antimicrobial therapies. Determination of secondary metabolites within the genome revealed seven different secondary metabolites distributed on 11 biosynthetic gene clusters. Various virulence factors were found, but the isolate was predicted to be a non-human pathogen.

Indexed as

Genome, BacterialPseudomonasTsetse FliesAnimalsBase CompositionCameroonPhylogenyWhole Genome SequencingAntimicrobial resistance genesComparative analysisPseudomonasSecondary metabolitesVirulence factorsWhole-genome sequencing

Identifiers

PMID41390370
PMCPMC12822146

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.