ArticleMolecular biology reports2025
Transcription factor ATF3 positively regulates the transcription of NSUN6 gene.
Article in Molecular biology reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
backgroundNSUN6 (NOL1/NOP2/sun 6) is an mRNA m5C methylation-modified methyltransferase, whose aberrant expression or mutation leads to many cancers. There are fewer studies related to the regulatory mechanisms of NSUN6 itself.
methodsA series of full-length and truncated plasmids of NSUN6 promoter region were constructed, and the core regulatory region of NSUN6 promoter was preliminarily characterized. Online website predicted the transcription factor binding sites in the core functional region, and the dual luciferase reporter gene assay, RT-qPCR, Western blot and ChIP assays confirmed the regulatory function of the transcription factor ATF3 on the NSUN6 promoter region.
resultsThe NSUN6 promoter full-length and truncation plasmids were successfully constructed, and it was determined that the -74 to -34nt region of the NSUN6 promoter is the core region for regulating the transcriptional activity of the gene. ATF3 is the key transcription factor regulating the activity of the NSUN6 promoter. The transcriptional activity of NSUN6 was significantly decreased after mutating the ATF3 binding site. Overexpression and knockdown of ATF3 resulted in a concomitant increase and decrease in the transcriptional activity, mRNA and protein levels of the NSUN6. Chromatin immunoprecipitation assays confirmed that the transcription factor ATF3 could bind to the NSUN6 promoter region in vivo. Also, an expression correlation between NSUN6 and ATF3 was observed in several tumors.
conclusionThe transcription factor ATF3 is a key transcription factor that positively regulates NSUN6 transcriptional activity. This provides new basic therapy and potential drug targets for NSUN6-related clinical diseases.
Indexed as
Identifiers
41389305What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.