ArticleBMC veterinary research2025
Development of an immortalized porcine intramuscular pre-adipocyte cell line to facilitate adipogenic investigation in vitro.
Article in BMC veterinary research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Intramuscular fat (IMF) deposition critically influences pork quality, necessitating reliable in vitro models to investigate its regulatory mechanisms. However, developing cost-effective porcine intramuscular pre-adipocyte (IMPA) seed cells capable of sustained proliferation and complete adipogenic differentiation remains technically challenging. Here, we established an immortalized porcine IMPA cell line through lentiviral-mediated transduction of simian virus 40 large T antigen (SV40T). This cell line retained differentiation capacity for over 25 passages, addressing limitations in primary cell lifespan. Functional evaluations demonstrated robust proliferative activity via CCK-8 assays, sustained telomerase activity, and minimal apoptosis, confirming stable growth characteristics during long-term culture. Furthermore, adipogenic induction assays revealed preserved differentiation potential, evidenced by lipid droplet accumulation and adipogenic marker expression. Collectively, this immortalized porcine IMPA cell line provides a valuable and reproducible model for elucidating molecular mechanisms underlying IMF deposition, offering significant utility for agricultural and biomedical research.
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