Evidence map›Paper›PMID 41387703›Full record

ArticleNature communications2025

A bright and regenerative array tag with fluorogenic ligands for long-term live-cell nanoscopy and single-molecule tracking.

Dongjuan Si, Zhihao Dong, Gang Xing, Longfang Yao, Jun Lu, Min Ma, Yue Yuan, Hanqing Zhao, Biqin Dong, Cong Li and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Dongjuan Si *School of Pharmaceutical Sciences, MOE Key Laboratory of Smart Drug Delivery, MOE Innovative Center for New Drug Development of Immune Inflammatory Diseases, Endoscopy Center and Endoscopy Research Institute, Zhongshan Hospital, Fudan University, Shanghai, 201203, China.
Zhihao Dong *School of Pharmaceutical Sciences, MOE Key Laboratory of Smart Drug Delivery, MOE Innovative Center for New Drug Development of Immune Inflammatory Diseases, Endoscopy Center and Endoscopy Research Institute, Zhongshan Hospital, Fudan University, Shanghai, 201203, China.
Gang Xing *School of Pharmaceutical Sciences, MOE Key Laboratory of Smart Drug Delivery, MOE Innovative Center for New Drug Development of Immune Inflammatory Diseases, Endoscopy Center and Endoscopy Research Institute, Zhongshan Hospital, Fudan University, Shanghai, 201203, China.
Longfang YaoSchool of Artificial Intelligence Science and Technology, University of Shanghai for Science and Technology, Shanghai, China.
Jun LuAcademy for Engineering and Technology, Yiwu Research Institute, Fudan University, Shanghai, China.
Min MaInterdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai, China.
Yue YuanInterdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai, China.
Hanqing ZhaoSchool of Pharmaceutical Sciences, MOE Key Laboratory of Smart Drug Delivery, MOE Innovative Center for New Drug Development of Immune Inflammatory Diseases, Endoscopy Center and Endoscopy Research Institute, Zhongshan Hospital, Fudan University, Shanghai, 201203, China.ORCID http://orcid.org/0000-0002-1037-4829
Biqin DongAcademy for Engineering and Technology, Yiwu Research Institute, Fudan University, Shanghai, China.ORCID http://orcid.org/0000-0003-2283-4498
Cong LiSchool of Pharmaceutical Sciences, MOE Key Laboratory of Smart Drug Delivery, MOE Innovative Center for New Drug Development of Immune Inflammatory Diseases, Endoscopy Center and Endoscopy Research Institute, Zhongshan Hospital, Fudan University, Shanghai, 201203, China.ORCID http://orcid.org/0000-0001-7731-8031
Lin YuanState Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, China. lyuan@hnu.edu.cn.ORCID http://orcid.org/0000-0002-1015-5319
Zhongwen ChenInterdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai, China. chenzw@sioc.ac.cn.ORCID http://orcid.org/0000-0001-5218-0152
Lu WangSchool of Pharmaceutical Sciences, MOE Key Laboratory of Smart Drug Delivery, MOE Innovative Center for New Drug Development of Immune Inflammatory Diseases, Endoscopy Center and Endoscopy Research Institute, Zhongshan Hospital, Fudan University, Shanghai, 201203, China. lwangfd@fudan.edu.cn.ORCID http://orcid.org/0000-0001-8412-2985

Funding

National Natural Science Foundation of China (National Science Foundation of China) 22477016
6 · The paper itself

Abstract

Live-cell fluorescence nanoscopy and single-molecule tracking offer profound insights into cellular biology, but their application is limited by fluorescent tags with low brightness, large size, and irreversible photobleaching. Here, we present ReTagX, a bright, regenerative, compact array tag system enabling rapid recovery of photobleached signals through reversible, fluorogenic labeling. Newly developed ReTag1 (~18 kDa) with highly fluorogenic MaP655-TMP achieves a signal-to-noise ratio up to 204-fold and allows fluorescence recovery within 1 minute in wash-free live-cell imaging. This tag extends STED imaging of mitochondrial TOM20 from fewer than 5 to over 300 frames, enabling long-term visualization of mitochondrial dynamics at 80 nm resolution for over 100 minutes. Multimeric ReTag1 arrays, such as ReTag8 (~156 kDa), increase brightness 5.5-fold and extend TNFR2 single-molecule tracking ~2.9-fold, preserving >80% of trajectories over 5 minutes. ReTagX provides a robust platform for high-resolution, long-term molecular visualization of cellular dynamics in living cells.

Indexed as

Fluorescent DyesNanotechnologySingle Molecule ImagingHeLa CellsHumansLigandsMicroscopy, FluorescenceMitochondriaFluorescent DyesLigands

Identifiers

PMID41387703
PMCPMC12808733

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.